[Assay of adenine nucleotide level in platelet storage pool for evaluation of the course of hemoblastosis].

The content of adenine nucleotides (ATP, ADP) was studied in dense granules of platelets in hemoblastosis to estimate the character of the pathological process course. Varying biochemical defects were observed at the levels of ATP and ADP depending on the severity of the pathological process in chronic myeloid leukemia, acute lymphoblastic leukemia, acute myeloblastic and acute myelo-monoblastic leukemias. Adenine nucleotide values can be used for the diagnosis of varying stages of the above diseases, for the evaluation of anomalous platelets and characterization of the adequacy of the bone marrow hemopoiesis.

[Importance of studying the level of platelet adenine nucleotide levels in various phases of chronic myeloleukoses].

Content of adenine nucleotides was studied in various compartments of blood platelets obtained from donors and patients with chronic myeloleukosis at various steps of the disease. Dissimilar biochemical defect was found in content of ATP and ADP, which varied in thrombocyte storage granules depending on severity of the disease. The data on adenine nucleotides content may be used for diagnosis of various steps of chronic myeloleukosis, for detection of thrombocytes anomaly and for evaluation of the state of medullary hemopoiesis.

[Comparative characteristics of the catalytic properties of platelet adenosine deaminase in normal states and in chronic myeloid leukemia].

Properties of adenosine deaminase from thrombocytes of healthy persons and of patients with chronic myeloleukosis were studied in presence of various structural analogues of purines and metal ions as well as at various temperature. Catalytic properties of the enzyme in thrombocytes of patients with chronic myeloleukosis were distinct from those of thrombocytes of healthy donors. These differences in the enzymatic properties (contrary to standard estimation of the enzyme activity) might be used both as a criterion of adenosine deaminase anomalies and for detection of the initial steps of the disease.

[The role of adenine nucleotides in the functional activity of platelets in hemophilia A].

Content of adenine nucleotides was studied in various pools of thrombocytes from donors and from patients with hemophilia A. Biochemical deficiency of adenine nucleotides appears to occur in the pool of thrombocytes obtained from patients with hemophilia A; estimation of ADP and ATP in the storage granules as well as of the nucleotides specific radioactivity may serve as a criterion of thrombocyte disfunction.

[Adenine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase of blood platelets in hereditary coagulopathies].

Activities of adenine phosphoribosyltransferase (EC 2.4.2.

[Platelet adenosine desaminase in various hematological diseases].

Activity of adenosine deaminase (EC 3.5.4.

[Thrombocyte purine nucleoside phosphorylase in various blood diseases].

Activity of purine nucleoside phosphorylase (EC 2.4.2.

[Thrombocyte adenase of healthy monkeys and monkeys with different degrees of hemoblastosis].

Sensitive radioactive method was used to study the activity of blood platelet adenase in healthy monkeys and in monkeys with hemoblastosis of different severity. A considerable increase of the specific activity of the enzyme in hemoblastosis with an expressed clinical picture and a reduction of the enzymatic activity at the stage of remission and in mild form of the disease was demonstrated. No adenase was revealed in the platelets of healthy monkeys.

[Adenase inhibitor in thrombocytes of healthy humans].

An inhibitor of adenase, which is absent in leukemic patients, was studied in thrombocytes of healthy persons. The inhibitor, localized in the granular fraction, was isolated by ultracentrifugation in sucrose gradient.

[Adenase of formed elements of blood of donors and of patients with different blood diseases].

The adenase activity was determined in erythrocytes, leucocytes and thrombocytes of donors and of patients with chronic lympholeucosis, chronic myeloleucosis and acute leucosis. A decrease in specific activity of the enzyme was observed in erythrocytes of the patients. In the leucocytes the adenase activity was not found both in normal persons and in all the patients examined.