Effect of estradiol on DNA and protein synthesis in rat liver in vivo.

The article studies the effect of 17, beta-estradiol on the binding of cytosol estradiol-receptor complex with chromatin isolated from the liver of female albino rats (sham-operated, ovariectomized and treated after the ovariectomy with 20 micron hormone/100 g body weight for 11 days) and sexually mature male albino rats. Higher binding of the complex is found for the female animals compared with the males. Parallel experiments are made to study the incorporation of 3H-thymidine and 14C-glycine in vivo, in acid-soluble and acid-insoluble liver fractions of female rats, respectively, which is used as a basis for assessing the rate of DNA and protein synthesis.

Significance of Cys-153 for the phosphatase activity of glyceraldehyde-3-phosphate dehydrogenase.

After oxidation of the catalytically active sulfhydryl group of Cys-149, glyceraldehyde-3 phosphate dehydrogenase is known to acquire an acylphosphatase activity. Modification of one cysteine residue, obviously Cys-153, with p-mercuribenzoate or N-ethylmaleimide fully inactivates the phosphatase activity of the oxidized enzyme suggesting that this amino acid residue is involved in the reaction. An acyl transfer between Cys-153 and the sulfenic acid derivative of Cys-149 in the mechanism of the phosphatase reaction could explain the data obtained.

[Sedimentation characteristics of the virions and subviral particles of influenza C viruses].

Investigation of sedimentation and some other properties of subviral particles and native virions of influenza C viruses was carried out in comparison with influenza A virions. Sedimentation rate of influenza C viruses was found to be 0.6 of that of influenza A viruses, the optical density peak coincided with the peaks of hemagglutinating and infectious activities of both influenza A and influenza C viruses.

Detection of influenza virus by means of immune sera to M and RNP inner proteins in solid phase radioimmunoassay.

Solid phase radioimmunoassay (SPRIA) was shown suitable for detection of influenza virus in various biological materials using immune sera (IS) to membrane protein (MP) and ribonucleoprotein (RNP) isolated from the strain A/USSR/90/77. Either serum was able to detect influenza A viruses with different haemagglutinins (H1, H3, H7) and did not react with influenza B viruses. SPRIA detected 0.

[Effect of a number of preparations on the function of the immune system in experimental influenza in mice].

(CBA X C57B1) X F1 mice were sensitized intraperitoneally with sheep erythrocytes and infected with influenza A viruses: nonpathogenic Leningrad-77 (H1N1) or pathogenic PR8 (HON1), before or five days after administration into the oesophagus of sodium succinate, levamisole, complexes I (panangin, sodium succinate, sodium glutamate) and 2 (lipoic acid, phosphothyamine, riboflavin, sodium pantothenate). The number of rosette-forming cells (RFC) in the spleen at 7 and 14 days postinfection, antibody titres, interferon level in the blood, the amount of virus in the lungs, spleen and lung morphology were studied. All the preparations used were found to increase the number of RFC in the spleen.

[Test system for detecting and differentiating the influenza virus by an immunoenzyme method].

Detection and differentiation of small amounts of influenza A and B viruses was done by enzyme-immunoassay based on detection of the complex of internal proteins. It was shown that two kinds of sera to the complex of internal proteins could be used: sera against disrupted viruses grown in a different system (mice or cell culture) and containing almost no CAM component for virus detection in the allantoic fluid of chick embryos, and sera to preparations of viruses grown in chick embryos and disrupted with detergents for elimination of virion surface determinants (wastes of subunit vaccine) for virus detection in nasal secretions of vaccinated subjects. The test-systems containing antibodies to the complex of internal proteins were shown to be as sensitive and specific as those containing antibodies to pure M protein and RNP.

[Comparative characteristics of the biophysical properties of influenza A, B and C proteins].

A comparative analysis of electrophoregrams of influenza A, B, and C virus proteins in polyacrylamide gel and in agarose was made. Separation of proteins was similar in three influenza C virus strains tested and differed from that of influenza A and B virus proteins. The possibility of preparative isolation of supercapsid and internal proteins of influenza C virus with preservation of their antigenic and immunogenic properties was demonstrated.

Fractionation of non allelic histone variants by hydrophobic interaction chromatography.

A method for the separation of non allelic histone variants is described. Sepharose-CL-4B was modified with dodecylamine by the carbonyldiimidazole-method. The matrix prepared contained 6 mumol/ml hydrophobic groups, C12-hydrocarbon chains.

[Accumulation of cholesterol in Acholeplasma laidlawii membranes in the steady-state phase of culture growth].

In early logarithmic phase of growth of the A. laidlawii cells the lipid composition of plasma membrane is changed: the total lipid, glycolipid and phospholipid contents are decreased, while that of cholesterol changes only insignificantly. In late logarithmic and steady state phases the cholesterol level in the membrane is increased in parallel with the decrease of the phospholipid content.

[Use of catabolic repression in the selection of the glucoamylase-producer Aspergillus niger].

The effect of various carbon sources and cAMP on the glucoamylase synthesis in Aspergillus niger was studied to find carbon sources repressed the enzyme synthesis and conditions for the selection of catabolite stable mutants. Maltose at a concentration of 0.5% stimulated the glucoamylase synthesis, but at a concentration of 4% it repressed not only the enzyme synthesis but the growth of the parental strain on the agar medium.

[Determination of the neuraminidase specificity of influenza A and B viruses using a modified lectin test].

A highly purified lectin was obtained from peanuts. The lectin test was modified for determination of the neuraminidase activity of influenza viruses. The test was found to be useful for the determination of the antigenic specificity of neuraminidase.

[Use of agarose for isolating influenza virus proteins retaining a high immunogenicity].

A method of influenza virus separation in agarose was modified. The possibility of using agarose of the national make and Difco agar was established. RNP and M proteins were isolated.

[Epidemiological analysis of the influenza virus A carrier state in healthy children].

Clinical and laboratory examinations in two permanently observed children's institutions were carried out in 1968--1978. Altogether, 241 children were examined virologically 759 times, of them 181 children were found to be truly healthy. In the epidemic period the latter yielded virus in 3.

Differentiation of influenza A virus nucleoproteins in enzyme-linked immunosorbent assay.

The serum antibody titre to the nucleoprotein (NP) of the influenza virus recombinant MRC-11 was determined in virus strains A/USSA/5/80 (H3N2), A/Hong Kong/8/64 (H3N2), A/duck/Ukraine/63 (Hav7Neq2) and in a recombinant strain between A/tern/Frunse/334/78(Hav4Nav1) and A/PR/8/34(H0N1) using the enzyme-linked immunosorbent assay (ELISA). Significant differences between the NP of these strains were found proving the usefulness for ELISA for such investigations.