Modified lateral enucleation technique-Surgery without ligation or clamping of the optic nerve: Technique description, complication rate and risk factors, and intraoperative blood loss estimation in companion animals.

Purpose: The purpose of this study is to describe modification of the lateral enucleation technique without ligation or clamping of the optic nerve, document the incidence of complications, estimate intraoperative blood loss and identify possible risk factors for the developments of complications.

Methods: Medical records of dogs, cats, and rabbits undergoing lateral enucleation without clamping of the optic nerve were identified and retrospectively reviewed for post-operative complications (2000-2022). The significance of possible risk factors for the development of complications, including species, sex, age, eye, surgeon, presumed ocular surface infection, cultures, follow-up, antibiotics, NSAIDs, complications and diabetes mellitus was examined in a subset of these patients (2019-2022).

[Development and progress of the National Autopsy Network (NATON)].

Background: Autopsies have long been considered the gold standard for quality assurance in medicine, yet their significance in basic research has been relatively overlooked. The COVID-19 pandemic underscored the potential of autopsies in understanding pathophysiology, therapy, and disease management. In response, the German Registry for COVID-19 Autopsies (DeRegCOVID) was established in April 2020, followed by the DEFEAT PANDEMIcs consortium (2020-2021), which evolved into the National Autopsy Network (NATON).

Determinants of public interest in emerging and re-emerging arboviral diseases in Europe: A spatio-temporal analysis of cross-sectional time series data.

Introduction: Climate change, the resulting geographical expansion of arthropod disease vectors, and increasing international mobility are contributing to the emergence of arboviral diseases in Europe. Public interest in vector-borne diseases and a subsequent gain of awareness and knowledge are essential to control outbreaks but had not yet been systematically assessed prior to this analysis.

Methods: Trends, patterns, and determinants of public interest in six emerging and re-emerging arboviral diseases were assessed in a spatio-temporal analysis of Google Trends data from 30 European countries between 2008 and 2020 while controlling for potential confounders.

Re-mining serum proteomics data reveals extensive post-translational modifications upon Zika and dengue infection.

Zika virus (ZIKV) and dengue virus (DENV) are two closely related flaviviruses with similar symptoms. However, due to the implications of ZIKV infections for pregnancy outcomes, understanding differences in their molecular impact on the host is of high interest. Viral infections change the host proteome, including post-translational modifications.

[Patient Preferences for Exercise Therapy in People with Severe Mental Illness in Germany].

Purpose: Assessing the experience with and the attitudes towards exercise therapy in persons with severe mental illness (SMI). Furthermore, potential variables of high preference towards exercise therapy are investigated.

Methods: Cross-sectional observational study of SMI patients aged between 18 and 65 years (n=385).

New Proteomic Signatures to Distinguish Between Zika and Dengue Infections.

Distinguishing between Zika and dengue virus infections is critical for accurate treatment, but we still lack detailed understanding of their impact on their host. To identify new protein signatures of the two infections, we used next-generation proteomics to profile 122 serum samples from 62 Zika and dengue patients. We quantified >500 proteins and identified 13 proteins that were significantly differentially expressed (adjusted p-value < 0.

Intracanalicular injection of N-acetylcysteine as adjunctive treatment for sialoceles in dogs: 25 cases (2000-2017).

Objective: To describe intracanalicular injection of 10% N-acetylcysteine (IINAC) as adjunctive treatment for sialoceles in dogs.

Animals: 25 client-owned dogs.

Procedures: Hard copy medical records at 2 veterinary ophthalmology practices were searched to identify dogs that underwent IINAC for treatment of sialoceles from January 2000 to December 2017.

First pass success of tracheal intubation using the C-MAC PM videolaryngoscope as first-line device in prehospital cardiac arrest compared with other emergencies: An observational study.

Background: Successful airway management is a priority in the resuscitation of critically ill or traumatised patients. Several studies have demonstrated the importance of achieving maximum first pass success, particularly in prehospital advanced airway management.

Objective: To compare success rates of emergency intubations between patients requiring cardiopulmonary resuscitation (CPR) for cardiac arrest (CPR group) and other emergencies (non-CPR group) using the C-MAC PM videolaryngoscope.

Ectopic Expression of Hematopoietic SHIP1 in Human Colorectal Cancer.

Colorectal cancer (CRC) is a heterogeneous disease that results from the accumulation of mutations in colonic mucosa cells. A subclass of CRC is characterized by microsatellite instability, which is thought to occur mainly through inactivation of the DNA mismatch repair genes and . The inositol 5-phosphatase SHIP1 is expressed predominantly in hematopoietic cells.

New insights into the cellular temporal response to proteostatic stress.

Maintaining a healthy proteome involves all layers of gene expression regulation. By quantifying temporal changes of the transcriptome, translatome, proteome, and RNA-protein interactome in cervical cancer cells, we systematically characterize the molecular landscape in response to proteostatic challenges. We identify shared and specific responses to misfolded proteins and to oxidative stress, two conditions that are tightly linked.

Effect of data self-collection as an activating teaching method in a statistical software course in medical biometry - a pilot study.

Biostatistics is an integral part of the studies of human medicine. Students learn the basics of analyzing and interpreting study results. It is important to demonstrate the subject's relevance by means of appropriate measures to maximize learning success.

Sample size estimation for pilot animal experiments by using a Markov Chain Monte Carlo approach.

The statistical determination of sample size is mandatory when planning animal experiments, but it is usually difficult to implement appropriately. The main reason is that prior information is hardly ever available, so the assumptions made cannot be verified reliably. This is especially true for pilot experiments.

A keratoprosthesis prototype for the dog.

Objective: To describe the technique for implantation of a novel keratoprosthesis (KP) prototype and evaluate its application for the treatment of corneal blindness in dogs.

Animals Studied: Seven dogs, all of them being clinically blind before surgery as a result of severe corneal endothelial disease (5/7) or chronic superficial keratitis (2/7) that were unresponsive to prior therapy.

Procedures: A silicone KP was implanted unilaterally, just anterior to Descemet's membrane, after creating a stromal pocket by deep stromal lamellar dissection.

Radiotherapy for canine chronic superficial keratitis using soft X-rays (15 kV).

Objective: To evaluate the effect of soft X-ray therapy in the treatment of refractory chronic superficial keratitis (CSK).

Animals Studied: Thirteen dogs with severe CSK, that had been refractory to prior medical and/or surgical therapy were treated with soft X-ray therapy.

Procedures: Both corneas of each dog were irradiated with soft X-rays (15 kV), to a total dose of 30 Gy, administered as two fractions over 48-96 h.

Aberrant conjunctival stricture and overgrowth in the rabbit.

Six dwarf rabbits with unilateral (n = 2) and bilateral (n = 4) aberrant conjunctival growth underwent surgical correction. The applied technique involved centrifugal incisions of the overgrowing conjunctiva to the limbus and a transpalpebral fixation of the conjunctiva. In this way the conjunctival fornix was reconstructed.

Clinical evaluation of pimecrolimus eye drops for treatment of canine keratoconjunctivitis sicca: a comparison with cyclosporine A.

The aim of this study was to evaluate the efficacy of pimecrolimus oil-based eye drops in alleviating the clinical signs of keratoconjunctivitis sicca (KCS) in dogs and to compare the efficacy with that of cyclosporine A (CsA) ointment. An open-label, multicenter study enrolling 44 dogs previously untreated with CsA was conducted. Dogs were randomly assigned to a treatment group and medicated twice daily for 8 weeks.

Further investigation of the tolerance and mechanical adjustability of the *Acri.Tec AR-1 PC/IOL in rabbit eyes: an intraocular lens with reversibly adjustable optical power.

Purpose: To examine the tolerance and mechanical function of an adjustable intraocular lens (IOL) in rabbit eyes.

Methods: Implantation of the *Acri.Tec AR-1 PC/IOL into 14 rabbit eyes.

Conjunctival dermoid in two guinea pigs: a case report.

Clinical and histologic findings of conjunctival dermoids in two unrelated guinea pigs are described. The dermoids were treated surgically by resection with superficial lamellar keratectomy and corneal epithelial debridement. Histologically the dermis underneath a stratified squamous keratinized and variably pigmented epithelium consisted of multiple hair follicles, with clearly visible arrectores pilorum muscles, sebaceous glands and loose vascularized fatty tissue.

Dacryops in a red-eared slider (Chrysemys scripta elegans): case report.

Clinical and histologic findings of an orbital lacrimal cyst (dacryops) in a 23-year-old-female red-eared slider (Chrysemys scripta elegans) are described. Main ophthalmologic findings included unilateral exophthalmus, engorged episcleral vessels, periocular swelling superior to the globe, incomplete lid closure, and reduced ocular motility. Based on sonographic and cytologic examination of the tissue, the preliminary diagnosis of an orbital cyst was established.

[Treatment of keratitis superficialis chronica of the dog with strontium 90].

Background: Corneal pannus is a disease which, if untreated, nearly always is progressive and may lead to blindness of the affected dog. A therapeutic standard is yet to be defined. Beta-ray irradiation with Sr-90 is often recommended on a casuistic basis, but systematic studies are sparse.

Persistent hyperplastic tunica vasculosa lentis and persistent hyperplastic primary vitreous (PHTVL/PHPV) in two cats.

Two domestic shorthair cats (6 and 9 months old) were presented for examination of ocular opacities. One cat had bilateral persistent pupillary membranes, unilaterally accompanied by persistent hyperplastic tunica vasculosa lentis, persistent hyperplastic primary vitreous (PHTVL/PHPV), and cataract. The second cat had bilateral PHTVL/PHPV with dense white plaques in the posterior lens capsule and subcapsular cortex.

Feline eosinophilic conjunctivitis.

Objective: To review 12 cases of histologically confirmed feline eosinophilic conjunctivitis, their clinical, cytologic, histologic and electronmicroscopic findings, results on PCR for FeHV-1, treatment and outcome.

Animals Studied: Twelve naturally occurring cases presented during a period of 26 months.

Procedures: Thorough ophthalmologic examination, conjunctival scrapings performed with the cytobrush method; histologic samples from the palpebral conjunctiva; PCR for FeHV-1 on Schirmer Tear Test (STT) strips; saliva and nasal swabs, and retrospective evaluation of all results.