The effect of laser preexposure on seeding endothelial cells to a biomaterial surface.

Objective: In this study, the possible effect of low-level laser (LLL) on improving the adhesion of endothelial cells (ECs) to a biomaterial substrate was evaluated.

Background Data: Despite the numerous studies regarding the effects of LLL on biologic systems, the influence of LLL on the binding between cells and materials was rarely investigated.

Materials And Methods: A low-power He-Ne laser apparatus with a continuous wavelength of 632.

Ultrasound preexposure improves endothelial cell binding and retention on biomaterial surfaces.

In spite of the extensive studies regarding the effects of ultrasound on biological systems, the influence of low-intensity ultrasound on endothelial cells has rarely been investigated. In this work, the effect of ultrasound in improving the binding between endothelial cells and biomaterial substrates was evaluated. Based on the results, low-intensity ultrasound could change the morphology and matrix secretion of endothelial cells, and such effects persisted when preexposed cells were seeded to another substrate.

The effect of dynamic culture conditions on endothelial cell seeding and retention on small diameter polyurethane vascular grafts.

Due to the limited number of cells available in endothelial cell (EC) seeding of small diameter vascular grafts, high seeding rate and ideal proliferation are normally required and can be achieved by optimizing the EC seeding and culture procedures. In this study, by using rotational seeding at 0.16 rpm for 12 h in an incubator, 90% cells were successfully seeded on the polyurethane vascular grafts.