Microwave assisted synthesis of negative-charge carbon dots with potential antibacterial activity against multi-drug resistant bacteria.

In this research, negative-charge carbon dots (CDs) were synthesized in one-step using a microwave and found to have potential antibacterial ability against multi-drug resistant bacteria. The CDs were synthesized by using citric acid and urea as precursors, and characterized by FT-IR, TEM and fluorescence spectrophotometry. The average size of CDs was about 2.

The Physiological Responses of Triggered by Phosphoribulokinase (PrkA) and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (Rubisco).

Phosphoribulokinase (PrkA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) have been proposed to create a heterologous Rubisco-based engineered pathway in for in situ CO recycling. While the feasibility of a Rubisco-based engineered pathway has been shown, heterologous expressions of PrkA and Rubisco also induced physiological responses in that may compete with CO recycling. In this study, the metabolic shifts caused by PrkA and Rubisco were investigated in recombinant strains where and genes (encodes phosphoenolpyruvate carboxylase and phosphate acetyltransferase, respectively) were deleted from MZLF ( BL21(DE3) Δ, Δ Δ).

Manipulating ATP supply improves in situ CO recycling by reductive TCA cycle in engineered .

The reductive tricarboxylic acid (rTCA) cycle was reconstructed in by introducing pGETS118KAFS, where (encodes α-ketoglutarate:ferredoxin oxidoreductase), (encodes ATP-dependent citrate lyase), (encodes fumarate reductase), and (encodes succinate dehydrogenase) were tandemly conjugated by the ordered gene assembly in (OGAB). MZLF ( BL21(DE3) Δ, Δ, Δ) was employed so that the C-2/C-1 [(ethanol + acetate)/(formate + CO)] ratio can be used to investigate the effectiveness of the recombinant rTCA for in situ CO recycling. It has been shown that supplying ATP through the energy pump (the EP), where formate donates electron to nitrate to form ATP, elevates the C-2/C-1 ratio from 1.

Exceeding the theoretical fermentation yield in mixotrophic Rubisco-based engineered Escherichia coli.

Rubisco-based engineered Escherichia coli MZLFB (E. coli BL21(DE3) Δzwf, Δldh, Δfrd) containing heterologous phosphoribulokinase (Prk) and Ribulose-1,5- bisphosphate carboxylase/oxygenase (Rubisco) was constructed for the mixotrophic growth. However, in situ CO recycling was hindered by clogs of pyruvate during glucose metabolism, which consequently resulted in an insufficient regeneration of NAD through the pflB-mediated ethanol production.

Bioreactors and in situ product recovery techniques for acetone-butanol-ethanol fermentation.

The microbial fermentation process is one of the sustainable and environment-friendly ways to produce 1-butanol and other bio-based chemicals. The success of the fermentation process greatly relies on the choice of bioreactors and the separation methods. In this review, the history and the performance of bioreactors for the acetone-butanol-ethanol (ABE) fermentation is discussed.

The immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus under nitrite stress.

The white shrimp Litopenaeus vannamei were challenged with tryptic soy broth (TSB)-grown Vibrio alginolyticus at a dose of 1 x 10(6) colony-forming units (cfu) shrimp(-1), and then placed in water containing concentrations of nitrite-N at 0 (control), 1.12, 5.15, 11.