Publications by authors named "I van Vlijmen-Willems"
The transmembrane protein claudin-1 is critical for formation of the epidermal barrier structure called tight junctions (TJ) and has been shown to be important in multiple disease states. These include neonatal ichthyosis and sclerosing cholangitis syndrome, atopic dermatitis and various viral infections. To develop a model to investigate the role of claudin-1 in different disease settings, we used CRISPR/Cas9 to generate human immortalized keratinocyte (KC) lines lacking claudin-1 (CLDN1 KO).
View Article and Find Full Text PDFThree-dimensional human epidermal equivalents (HEEs) are a state-of-the-art organotypic culture model in preclinical investigative dermatology and regulatory toxicology. In this study, we investigated the utility of electrical impedance spectroscopy (EIS) for noninvasive measurement of HEE epidermal barrier function. Our setup comprised a custom-made lid fit with 12 electrode pairs aligned on the standard 24-transwell cell culture system.
View Article and Find Full Text PDF3 D human epidermal equivalents (HEEs) are a state-of-the-art organotypic culture model in pre-clinical investigative dermatology and regulatory toxicology. Here, we investigated the utility of electrical impedance spectroscopy (EIS) for non-invasive measurement of HEE epidermal barrier function. Our setup comprised a custom-made lid fit with 12 electrode pairs aligned on the standard 24-transwell cell culture system.
View Article and Find Full Text PDFBackground: Interleukin (IL)-13 is a key driver of inflammation and barrier dysfunction in atopic dermatitis (AD). While there is robust evidence that tralokinumab - a monoclonal antibody that neutralizes IL-13 - reduces inflammation and clinical disease activity, less is known about its effects on barrier function.
Objectives: To characterize the effects of tralokinumab treatment on skin barrier function.
Article Synopsis
- The aryl hydrocarbon receptor (AHR) is crucial for maintaining skin cell homeostasis and function as an environmental sensor.
- Upon activation by ligands, AHR influences gene expression by binding to open chromatin, which leads to the swift activation of transcription factors like TFAP2A.
- This study reveals the AHR-TFAP2A pathway's role in promoting keratinocyte differentiation and skin barrier formation, offering new insights for treating skin barrier-related conditions.