[Ubiquitous downregulation of InR gene expression affects stress associated hormone metabolism in Drosophila females].

The effect of the ubiquitous downregulation of insulin receptor (InR) gene expression on the metabolism of juvenile hormone (JH) and dopamine (DA) in young females of D. melanogaster under normal conditions and heat stress is studied. The activity ofJH degradation and alkaline phosphatase (ALP, an enzyme regulating DA synthesis) were used as indicators of JH and DA levels, respectively.

[Effect of sexual maturation on DD2R gene expression in fat body of Drosophila melanogaster females].

The expression of the DD2R gene was studied by in situ hybridization in the fat body (place of the synthesis of enzymes that degrade juvenile hormone) and ovarian follicular cells (place of the synthesis of 20-hydroxyecdysone) in young and sexually mature D. melanogaster females. It was demonstrated that the DD2R gene is expressed in the fat body, and its expression is higher in young females than in sexually mature females.

[Role of DopR in the molecular mechanism of the dopamine control of juvenile hormone metabolism in female Drosophila].

The effect of a decreased availability of the D1-like dopamine receptor (DopR) in Drosophila (caused by DopR antagonist added into food) on the juvenile hormone (JH) synthesis rate in young female D. melanogaster has been studied. The JH degradation rate and the alkaline phosphatase (ALP) and tyrosine decarboxylase (TDC) activities were used as indicators of the JH synthesis rate.

[The DD2R gene expression level in the corpus allatum affects the fitness of female Drosophila melanogaster].

The effect of tissue-specific suppression of the dopamine D2-like receptor gene (DD2R) in the corpus allatum (CA), the gland that synthesizes juvenile hormone (JH) on the Drosophila melanogaster resistance to heat stress has been studied. A decreased expression of the DD2R gene in the CA has been found to substantially decrease the heat stress resistance of adult transgenic female, but not male, D. melanogaster compared to the control group, this phenomenon being weakly pronounced in juvenile flies.

[Inhibition of DD2R gene expression in the corpus allatum activates alkaline phosphatase in female Drosophila melanogaster].

Tissue-specific inhibition of the expression of the D2-like dopamine receptor gene (DD2R) in the corpus allatum (CA), which is a gland that synthesizes the juvenile hormone (JH), was tested for effect on alkaline phosphatase (AP) activity and the intensity of the AP response to heat stress (stress reactivity) in female Drosophila melanogaster. AP activity and AP stress reactivity in transgenic females with lower DD2R expression in the CA were higher than in control flies. A pharmacological elevation in JH increased AP activity in females of the control strains.