Publications by authors named "I Yoshinaga"

Article Synopsis
  • Underreporting in dietary surveys among individuals with obesity is a significant issue, particularly within the Japanese demographic, where data is limited.
  • This study analyzed the relationship between energy intake to basal metabolic rate (EI/BMR) and ketone body levels in 91 obese Japanese women, finding that 26.4% were classified as underreporters and 12.1% as energy-restricted reporters based on these measurements.
  • Results indicated that a low EI/BMR was linked to high levels of ketones, suggesting that combining EI/BMR with ketone body measurement could help identify underreporting in dietary surveys.
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While cyst germination may be an important factor for the initiation of harmful/toxic blooms, assessments of the fluctuation in phytoplankton cyst germination, from bottom sediments to water columns, are rare in situ due to lack of technology that can detect germinated cells in natural bottom sediments. This study introduces a simple mesocosm method, modeled after previous in situ methods, to measure the germination of plankton resting stage cells. Using this method, seasonal changes in germination fluxes of toxic dinoflagellates resting cysts, specifically Alexandrium fundyense (A.

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Article Synopsis
  • Bradyrhizobium sp. S23321 is a bacterium from paddy field soil that is closely related to the legume symbiont Bradyrhizobium japonicum USDA110 but does not induce root nodulation in siratro.
  • Its genome consists of a single circular chromosome with over 6,800 protein-encoding genes, but lacks the symbiosis islands and chaperonin gene cluster crucial for nodulation present in USDA110.
  • S23321 possesses a nitrogen fixation (nif) gene cluster similar to that of photosynthetic bradyrhizobia, alongside genes for a complete photosynthetic system and various transporters, suggesting its adaptability to low-nutrient environments.
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An anammox assay involving a ¹⁵N tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N₂ production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showed that the DNA in the top layer contained sequences related to those of Candidatus 'Brocadia fulgida', Ca.

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Twenty-five replicates of growth medium for anaerobic ammonium oxidation (anammox) containing (15)N-labeled ammonium and non-labeled nitrite were inoculated into an anammox enrichment culture at low density, and anaerobically incubated batchwise. In the headspace, (29)N(2) partial pressure linearly increased via anammox in 25 vials, confirming that anammox populations were viable in all subcultures. On prolonged incubation, exponential increases in (29)N(2) were not observed in all but 13 subcultures, suggesting that the anammox population may not proliferate unless all conditions for growth are satisfied.

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