[Value of using polyethylene glycol in the indirect antiglobulin test with erythrocytes].

A series of comparative investigations was carried out for establishing the value of the indirect antiglobulin test (IAT) in its first phase. The results showed that PEG, in relation to NaCl solution of low ionic strength, increased the reactivity of various antibodies of different specificity present in 72 studied sera. A significant superiority of IAT-PEG to IAT-BISS was noted in 45.

[Remarks on routine use of the enzyme test LEN (LISS Enzyme) in immunohematological studies].

The authors report one-year experience with the enzymatic test LEN (LISS-Enzym) replacing the one-step papain test in the compatibility testing, and in place of the two-step papain test in the detection and identification of red cells allo-antibodies. A wide range of investigations confirmed the usefulness of this test in the detection and antibodies specificity, mainly from the Rh system which are often undetectable by means of the indirect antiglobulin tests. It is concluded that in compatibility tests the LEN test should replace the low-sensitivity one-step papain test used as yet.

Analysis of immune response to red blood cell antigens in multitransfused patients with different diseases.

The rate of alloimmunization to red blood cell antigens in 1502 multitransfused patients, mainly with blood disorders, was analyzed in a retrospective study. The overall incidence of alloantibodies was 5.7%.

[Value of the LEN (LISS-enzyme) technic in detection of alloantibodies to erythrocytes].

The reported study was done for assessment of the value of the enzymatic technique LSE/LISS Spin Enzyme proposed by Odell et al. using a solution of NaCl of low ionic strength (LISS) for detection of incomplete alloantibodies to erythrocytes, especially those from the Rh system. The results of the study demonstrated that the LSE test, called by the authors LEN (LISS-Enzyme) is a sensitive and specific method comparable in its effectiveness to the two-step papain test (2-Pap) used widely in this country.