[The design of a diagnostic test system based on magnetic sorbents and liposomes].

The results obtained in the study of the possibility of using magnetic sorbents for the construction of a diagnostic assay system based on the antigen-antibody interaction are presented. As a model, Yersinia pestis capsular antigen and immunoglobulins to it have been used. A solid-phase immunofluorescent liposomal assay method has been developed; this method can be used for the detection of biopolymers in the sample under study and for the determination of their activity.

[Immunofluorescence method of detecting cholera enterotoxin].

The quantitative immunofluorescent assay for the determination of cholera enterotoxin is proposed. The assay is based on the selective sorption of cholera enterotoxin by gangliosides incorporated into polyacrylamide granules. The preliminary treatment of gangliosides with neuraminidase enhances the sensitivity of this assay.

[Use of neuraminidase for improving the properties of the erythrocytic ganglioside diagnosticum].

In this work the possibility of using neuraminidase for increasing the content of ganglioside GM1 in the mixture of gangliosides used for the sensitization of erythrocytes has been studied. The study has revealed that the treatment of gangliosides with neuraminidase is sufficient for obtaining active hemosensitin; there is no need for the purification of the preparation by gel filtration.