Assessment of alginate hydrogel degradation in biological tissue using viscosity-sensitive fluorescent dyes.

The main goal of this study is to investigate a combination of viscosity-sensitive and viscosity-insensitive fluorescent dyes to distinguish different rheological states of hydrogel based biostructural materials and carriers in biological tissues and to assess their corresponding location areas. The research is done in the example of alginate hydrogel stained with viscosity-sensitive dyes Seta-470 and Seta-560 as well as the viscosity-insensitive dye Seta-650. These dyes absorb/emit at 469/518, 565/591 and 651/670 nm, respectively.

[Cryosurgery in diffuse hepatic diseases].

Comparative studying, using histological and biomicroscopic methods, of the dosed cryohepatodestruction (CHD), periarterial cryodenervation of hepatic artery (CDHA) and their concomitant application influence on the dynamics of hepatic restoration processes in experimental cirrhosis was performed. The investigations were done on 215 male rats owing body mass 200-280 g in a not changed and pathologically changed liver. There was shown, that CDHA promotes changes in hepatic tissue microhemocirculation, as well as the enhancement of the sinusoidal vessels diameter and relative square of vascular bed.

Use of fractal [corrected] analysis for evaluation of liver structure and function in rats in vivo.

Fractal [corrected] and morphometrical analysis of images obtained by life-time photo- and videorecording of microhemocirculatory changes in the rats in health, experimental cirrhosis, and variants of its treatment was carried out. Differences in the fractional dimensions of the studied organs were significant and correlated with morphological values. This suggests fractal [corrected] analysis for the diagnosis and prediction of hepatic tissue status in vivo.

[The possibility of correcting metabolic disorders in experimental liver cirrhosis by cryodestruction and plasma-flow resection].

The first week after the cryodestruction, the resection of plasma flow and combined effect of the two methods causes metabolic changes, which later according to observations are normalized. The metabolic disturbances, which are connected with experimental liver cirrhosis under the combined cryodestructive effect and plasma flow resection are normalized three weeks after treatment. But there is no elimination of defects of free radical oxidation and there remains a decreased insulin binding capability of lymphocytes.