Publications by authors named "I V Nazarenko"
Article Synopsis
- * In a multicenter study involving 6,740 patients, a 16.5% incidence of SSIs was observed, with the majority being superficial, and many cases detected after hospital discharge; key risk factors included the type of surgical approach and operation duration.
- * The main pathogens identified were Escherichia coli and Staphylococcus aureus, with significant rates of antibiotic resistance noted, indicating a need for improved preventive measures to reduce complications post-surgery.
Article Synopsis
- Extracellular vesicles (EVs) show promise as biomarkers for liquid biopsy, but current methods struggle to isolate them effectively from complex biofluids like plasma and serum.
- Traditional isolation techniques often lead to contamination with lipoproteins, making it difficult to accurately assess the number and content of EVs.
- The introduction of Fast Protein Liquid Chromatography (FPLC) with Superose 6 offers a better solution to isolate small EVs while minimizing contamination, highlighting their potential for detecting disease-associated biomarkers, especially in pancreatic cancer patients.
Article Synopsis
- * Findings show high genetic diversity in the Kazakh Tobet, classified into seven distinct genetic clusters, indicating its complex genetics.
- * The research suggests shared ancestry with breeds like the Central Asian Shepherd Dog and Turkish Akbash, and highlights the breed's ancient origins, providing a foundation for preservation and international recognition.
Limbal epithelial progenitor cells (LEPC) rely on their niche environment for proper functionality and self-renewal. While extracellular vesicles (EV), specifically small EVs (sEV), have been proposed to support LEPC homeostasis, data on sEV derived from limbal niche cells like limbal mesenchymal stromal cells (LMSC) remain limited, and there are no studies on sEVs from limbal melanocytes (LM). In this study, we isolated sEV from conditioned media of LMSC and LM using a combination of tangential flow filtration and size exclusion chromatography and characterized them by nanoparticle tracking analysis, transmission electron microscopy, Western blot, multiplex bead arrays, and quantitative mass spectrometry.
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