Publications by authors named "I V Mindukshev"
Introduction: The recently developed platelet aggregation technique based on low-angle light scattering (LaSca) in diluted platelet-rich plasma (PRP) requires only a small sample volume and provides information about platelet aggregation and shape change. This study aimed to investigate the influence of preanalytical and analytical variables and to validate the method in a real-life pediatric hematology hospital setting.
Methods: Platelet aggregation was induced by ADP in diluted PRP in the presence of 2 mM calcium at 23 °C.
Article Synopsis
- - The study investigates the effects of decaffeinated green tea extract (GTE) and two ammonium salts (ACL and ACR) on physical performance in a rat swimming model, analyzing various biological and performance metrics.
- - The GTE + ACR combination showed the most significant improvements in swimming duration and lower lactate levels compared to the negative control group, indicating enhanced endurance.
- - While GTE helps in body recovery and immune response, ammonium carbonate specifically boosts physical performance. However, combining GTE with ammonium salts does not yield a clearly beneficial effect.
The ammonia/ammonium (NH/NH, AM) concentration in human erythrocytes (RBCs) is significantly higher than in plasma. Two main possible mechanisms for AM transport, including simple and facilitated diffusion, are described; however, the driving force for AM transport is not yet fully characterized. Since the erythroid ammonium channel RhAG forms a structural unit with anion exchanger 1 (eAE1) within the ankyrin core complex, we hypothesized the involvement of eAE1 in AM transport.
View Article and Find Full Text PDFSemi-anadromous animals experience salinity fluctuations during their life-span period. Alterations of environmental conditions induce stress response where catecholamines (CA) play a central role. Physiological stress and changes in external and internal osmolarity are frequently associated with increased production of reactive oxygen species (ROS).
View Article and Find Full Text PDFPathologies such as malaria, hemorrhagic stroke, sickle cell disease, and thalassemia are characterized by the release of hemoglobin degradation products from damaged RBCs. Hematin (liganded with OH) and hemin (liganded with Cl)-are the oxidized forms of heme with toxic properties due to their hydrophobicity and the presence of redox-active Fe. In the present study, using the original LaSca-TM laser particle analyzer, flow cytometry, and confocal microscopy, we showed that both hematin and hemin induce dose-dependent RBC spherization and hemolysis with ghost formation.
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