[Temperature denaturation of erythrocyte spectrin: rheology, deformability and resistance to detergents].

Detergent resistance of erythrocytes and viscosity of whole blood altered sharply already on the first stages of spectrin denaturation process (46-48 degrees C). Alternatively, deformability changes arise above 50 degrees C. Further insignificant increase in viscosity proceeded above 50 degrees C.

[Autohemolysis of human erythrocytes in the temperature range 4-37 C].

It is shown that the value of activation energy of red blood cells autohemolysis in temperature range of 4 + 37 degrees C is considerably smaller than that at t > 37 degrees C and is about 50 kJ/mol in isotonic saline buffer, pH 7.4. This means that the change of limiting stage of hemolysis occurs at 37 degrees C.

[Hemolysis of human erythrocytes by hemin. The role of hemin in erythrocyte autohemolysis].

Hemin hemolysis of human erythrocytes at 37 degrees C has been investigated by the kinetic method. It has been observed that hemin affects erythrocytes by two mechanisms, inducing "quick" and "slow" hemolysis. The "quick" hemolysis is completed after the first few minutes.

[Thermohemolysis of erythrocytes in the temperature range including physiologic (autohemolysis)].

The activation energy of thermohemolysis of erythrocytes changes from 36 +/- 5 kcal/mol (35-45 degrees C) to 97 +/- 5 kcal/mol (45-55 degrees C) at the temperature about 45 degrees C in isotonic buffer. The break on Arhenius' plot is preserved also when erythrocytes are placed into plasma. The character of Arhenius' plot is the same when erythrocyte hemoglobin is totally oxidated into methemoglobin by chemical way, though thermal stability of such erythrocytes is decreased.

[Thermohemolysis of erythrocytes. Model representations and mathematical processing of kinetic curves].

A mathematical model of erythrocyte thermohemolysis valid also for other types of hemolysis with S-shape kinetics is proposed. A formula is derived which, when entered into the computer programme, allows to obtain the distribution of erythrocytes according to the parameter reflecting their thermostability automatically from the experimental kinetic curve of erythrocyte thermohemolysis.