A comprehensive contribution of genes for aryl hydrocarbon receptor signaling pathway to hypertension susceptibility.

Objective: The present study was designed to investigate whether genetic polymorphisms of the aryl hydrocarbon receptor (AHR) signaling pathway are involved in the molecular basis of essential hypertension (EH).

Methods: A total of 2160 unrelated Russian individuals comprising 1341 EH patients and 819 healthy controls were recruited into the study. Seven common AHR pathway single-nucleotide polymorphisms (SNPs) such as rs2066853, rs2292596, rs2228099, rs1048943, rs762551, rs1056836, and rs1800566 were genotyped by TaqMan-based allele discrimination assays.

Association of Flavin Monooxygenase Gene E158K Polymorphism with Chronic Heart Disease Risk.

We studied the relationship between the risk of chronic heart disease and FMO3 gene polymorphism E158K analyzed by PCR and restriction fragment length polymorphism (RFLP) analysis. The homozygous 158KK genotype of FMO3 gene is associated with high risk of chronic heart disease in women, but not in men. FMO3 gene polymorphism E158K is a significant predictor of predisposition to chronic heart disease in women.

[Association of T174M polymorphism of the angiotensinogen gene with the higher risk of cerebral stroke in women].

Aim: To study the association of M235T (rs699) and T174M (rs4762) polymorphisms of the angiotensinogen (AGT) gene with the risk of cerebral stroke (CS) in the Russians of the Central Chernozem Region.

Materials And Methods: A total of 638 DNA samples obtained from 353 patients with CS and 285 sex- and age-matched healthy individuals were examined. The polymorphisms were genotyped by polymerase chain reaction (T174M) and TaqMan allelic discrimination (M235T) assays.

[Catalase gene polymorphism is associated with increased risk of cerebral stroke in hypertensive patients].

Experimental and clinical studies suggest that oxidative stress is an important pathogenetic mechanism of both essential hypertension (EH) and cerebral stroke (CS). In this paper, we investigated, for the first time, the relationship between polymorphisms of two genes for antioxidant defense enzymes such as catalase (CAT) and flavin-containing monooxygenase 3 (FMO3) and the risk of CS in hypertensive patients. DNA samples obtained from 667 unrelated Russian patients with EH (306 patients with CS, and 361 patients without cerebrovascular accidents) were genotyped for polymorphisms -21A>T (rs7943316) of the CAT gene and E158K (rs2266782) of the FMO3 gene.

[Are we controlling the sedation in ICU? A multicenter study results].

The aim of this study is to evaluate the issues of sedation and analgesia in all-purpose ICUs in Russia. To obtain that, a single-day observational survey was performed in 55 ICUs of Ural and Siberia regions. This work enabled to describe the targets, instruments of control and patterns of sedative and analgetics and sedatives prescription, as well as to make conclusions about issues in this area and possibilities of creation and necessity of analgesia and sedation standards.