[Myeloperoxidase activity in blood plasma as a criterion of therapy for patients with cardiovascular disease].

A significant increase in the myeloperoxidase (MPO) activity has been found in plasma of patients with stable angina and with acute coronary syndrome (ACS) in comparison with the control group. MPO concentration was significantly increased in plasma of ACS patients. Reduced MPO activity in the treated ACS patients correlated with a favorable outcome of the disease.

[Antioxidant status and glutathione redox potential of erythrocytes in patients with acute coronary syndrome].

Indicators of oxidative stress (OS), systemic inflammation, metabolism and redox status of glutathione (GSH) were investigated and compared in patients with ST-segment elevation myocardial infarction on electrocardiograms (STEMI), and patients with unstable angina (UA). The elevated and decreased myeloperoxidase level, superoxide dismutase activity, and moderate increased plasma levels of interleukin-6, while maintaining the antioxidant potential, were found in Group 1. Disorders in pro-/antioxidant balance and systemic inflammatory response were manifested in UA.

Measurement of plasma hemoglobin peroxidase activity.

We described a spectrophotometric method for measuring hemoglobin peroxidase activity in human plasma using o-dianisidine (o-DA) as the substrate and myeloperoxidase specific inhibitor 4-aminobensoic acid hydrazide (ruling out the probable contribution of myeloperoxidase to the measured parameter value). The optimal conditions (pH 5.5; 2 mM H2O2) have been determined, at which hemoglobin makes the main contribution to plasma oxidation of o-DA.

Functional activity of neutrophils in diabetes mellitus and coronary heart disease: role of myeloperoxidase in the development of oxidative stress.

We performed a comparative analysis of functional activity of neutrophils in patients with type 2 diabetes mellitus with and without symptoms of CHD. Enhanced H2O2 production by neutrophils in response to N-formyl-Met-Leu-Phe (fMLP) was found in patients with type 2 diabetes mellitus. In patients with type 2 diabetes mellitus associated with CHD, fMLP-induced release of myeloperoxidase from azurophilic granules of neutrophils was reduced and plasma myeloperoxidase level was elevated.

Lectin-induced aggregates of blood cells from patients with acute coronary syndromes.

Background: Cell surface glycoligands and circulating glycoproteins are believed to contribute to the pathogenesis of acute coronary syndromes (ACS) through cell aggregation/adhesion mechanisms. To characterize the glycobiological status of blood cells from patients with ACS, we used an advanced lectin-mediated aggregation technique allowing for detection of not only conventional lectin-induced cell aggregates but also their fraction resistant to haptenic/inhibitory sugars.

Methods: Peripheral blood samples were obtained from 24 patients with acute myocardial infarction or unstable angina and 18 healthy control subjects.