Aim: Analysis of antibiotic resistance profiles of Vibrio cholerae O1 strains isolated from the environmental objects in the territory of Russia in 2005 - 2012.
Materials And Methods: Antibiotocograms of 52 strains of V. cholerae were determined by serial dilution method in dense nutrient medium.
Zh Mikrobiol Epidemiol Immunobiol
September 2013
Aim: Evaluation of quality indicators of constructed cholera antigen polymer diagnosticums by using a complex of specific anti-cholera sera. MATERIALS AND METHODS. Cell lysates of cholera vibrio strains Vibrio cholerae cholerae 1395, V.
View Article and Find Full Text PDFThe article considers, the issue of producing the species-specific fluorescent monoclonal immunoglobulins to detect comma bacillus of serogrups O1 and O139 in the reaction of direct immunfluorescence. It is established that not only ascitic but culture fluid too can be the source of monoclonal antibodies for producing fluorescent conjugates. The optimal conditions are selected to produce the fluorescent monoclonal immunoglobulins-monoclonal antibodies.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
January 2012
Aim: Genotype characteristic and determination of serological properties of Vibrio cholerae nonO1/nonO139 strains that caused diseases in population of Rostov region from 2000 to 2009.
Materials And Methods: 15 clinical strains of V. cholerae nonO1/nonO139 were studied.
Aim: Determination of serogroup and PCR-genotyping of Vibrio cholerae non-O1/non-O139 strains isolated from surface basins and sewages of Rostov-on-Don city in 2003 - 2008.
Materials And Methods: Seven hundred strains of V. cholerae non-O1/non-O139 serogroups were studied in reaction of slide-agglutination with array of 80 diagnostic sera for non-O1/non-O139 serogroups.