The differential formation and composition of leukocyte-platelet aggregates induced by various cellular stimulants.

Background: Leukocyte-platelet aggregates comprise a pathogenic link between hemostasis and immunity, but the prerequisites and mechanisms of their formation remain not understood.

Aims: To quantify the formation, composition, and morphology of leukocyte-platelet aggregates in vitro under the influence of various cellular activators.

Methods: Phorbol-12-myristate-13-acetate (PMA), lipopolysaccharide (LPS), thrombin receptor-activating peptide (TRAP-6), and adenosine diphosphate (ADP) were used as cellular activators.

Protease activated receptor-4: ready to be part of the antithrombosis spectrum.

Purpose Of Review: Cardiovascular disease is a major cause of death worldwide. Platelets play a key role in this pathological process. The serine protease thrombin is a critical regulator of platelet reactivity through protease activated receptors-1 (PAR1) and PAR4.

Activation of Piezo1 channels in compressed red blood cells augments platelet-driven contraction of blood clots.

Background: Piezo1 is a mechanosensitive cationic channel that boosts intracellular [Ca]. Compression of red blood cells (RBCs) during platelet-driven contraction of blood clots may cause the activation of Piezo1.

Objectives: To establish relationships between Piezo1 activity and blood clot contraction.