[Nuclear ultrastructure of binuclear and trinuclear hepatocytes].

Binucleated and less frequently trinucleated hepatocytes appear in human and animal livers under normal conditions as well as in pathological cases. To provide further ultrastructural data hepatocyte nuclei were investigated in different pathological states. Important results of this study are: Size of the nuclei and structure of the karyoplasm as well as size and localization of the nucleolus are obviously, same in most of the binucleated hepatocytes.

[Ultrastructure of amitosis and mitosis of the liver].

Thousands of liver cells of several ultrastructural investigation programs of our laboratory have been evaluated in respect to amitosis and mitosis. Multiplication of hepatocytes by amitosis and mitosis often leads to bi-, tri- and multinucleated cells. No striking ultrastructural changes have been observed in the hepatocytes of human and animal livers.

Primary phase of hepatocytic autophagocytosis under ischaemic conditions.

Increased autophagocytosis in hepatocytes was found in response to conditions of ischaemia/hypoxia. Initial stages proved to be recordable. These were found to become manifest through the formation of phospholipid membrane structures, approximately 5 nm in width, coalescing in circular formations with vesicular extensions.

Electron microscopy of liver in patients with chronic haemodialysis.

Liver biopsies were performed on 18 haemodialysis patients with clinically as well as clinico-chemically altered liver values and partially positive HBV marker serology, with the view to comparing these values with ultrastructural findings in correlation with histological and immunohistological results. The following characteristic combination of findings was recorded at ultrastructural level: hypertrophy and hyperplasia of agranular endoplasmic reticulum of hepatocytes, haemosiderin deposits in Kupffer cells and hepatocytes, invasion of lymphocytes into Disse and intercellular spaces; megamitochondria in hepatocytes and perisinusoidal fibrosis established in some cases. The same findings proved to be recordable in histological diagnosis of hepatitis or toxico-metabolic liver damage.

Changes in the nucleotide metabolism of Ehrlich ascites tumour cells during their growth in vivo.

During the transition of Ehrlich mouse ascites tumour cells from the proliferating into the resting phase of growth a tremendous loss of purine and pyrimidine compounds was quantitated by ion-pair reversed-phase high performance liquid chromatography. This change is accompanied by a distinct decline in the incorporation rates of adenine, hypoxanthine, and adenosine. Inorganic phosphate stimulates the low rate of hypoxanthine incorporation of cells in the plateau phase, but lacks any effect on the high rate during proliferation.