Solid-phase antigen immunoassay for the detection of anti-zona pellucida-antibodies in clinically defined sera.

A new solid-phase antigen immunoassay was developed for the detection of anti-zona pellucida-antibodies. The assay was validated in a double blind study with clinically defined sera. The new assay is easy to perform and large numbers of sera can be processed in one day.

[Radioimmunological differentiation of yeasts (author's transl)].

A microtiter solid phase radioimmunoassay is described for the differentiation or microorganisms by using six species of yeasts as a model. Rabbits were immunized with homogenized cells of the yeasts. The rabbit sera obtained were absorbed to homologous cells and after elution, the antibodies so obtained were labeled with 125I.

[Study on the effect of formaldehyde on the immunological reactivity and morphology of hepatitis B-antigen (author's transl)].

Treatment of purified HB-antigen with formaldehyde solution changes its immunological reactivity and its morphology. A decrease in antigen activity by formaldehyde treatment could be demonstrated by radial immunodiffusion and by radioimmunoassay (Fig. 1).

Epidemiology of hepatitis B surface antigen (HBsAg) and antibody to HBsAg in hospital personnel.

The epidemiology of hepatitis B in hospital personnel was studied by testing of sera from 3,770 employees of the Medical School of Hannover (Hannover, West Germany) for hepatitis B surface antigen (HBsAg) and its corresponding antibody (anti-HBs) by solid-phase radioimmunoassay. An average prevalence of 2.2% for HBsAg and 11.