Catalytic enzyme activity concentration in tissues of man, dog, rabbit, guinea pig, rat and mouse. Approach to a quantitative diagnostic enzymology, III. Communication.

The catalytic activity of up to fifteen enzymes was investigated in the liver, heart, skeletal muscle, kidney (medulla, cortex), brain, lung, duodenum, spleen and pancreas from man and animals. Human specimens were obtained from autopsies and immediately post-mortem from dogs, rabbits, guinea pigs, rats and mice. The differences between our results and previous reports of considerably lower activities for structural enzymes (e.

Enzyme release from the perfused rat heart. The functions of the cytoskeleton under cell-pathological conditions.

The mechanism of enzyme release from Langendorff-perfused rat hearts was studied under the injury conditions of the Ca2+ paradox and 2,4-dinitrophenol poisoning. During perfusion with Krebs-Ringer buffer or in buffered sucrose sarcoplasmic enzymes were massively released when Ca2+ was reintroduced to the perfusion medium (Ca2+ paradox). Mitochondrial matrix enzymes were released to a very small extent.

Catalytic enzyme activity concentration in thoracic duct, liver, and intestinal lymph of the dog, the rabbit, the rat and the mouse. Approach to a quantitative diagnostic enzymology, II. Communication.

In the mixed body lymph of the thoracic duct and in the defined organ lymph of the liver and the intestine, the catalytic activity concentrations of up to sixteen enzymes and the concentrations of albumin and protein were determined, as well as the transport rate of these substances and their lymph/plasma ratio. Thoracic duct lymph specimens were obtained from an extracorporeal lymph shunt in anaesthetized and conscious dogs and from short-term fistulas in anaesthetized rabbits, rats and mice. Additionally, rabbits and rats underwent passive motion of the hind limbs in another experimental trial.

Catalytic enzyme activity concentration in plasma of man, sheep, dog, cat, rabbit, guinea pig, rat and mouse. Approach to a quantitative diagnostic enzymology, I. Communication.

For the most convenient laboratory animals in experimental medicine such as the sheep, dog, cat, rabbit, guinea pig, rat and mouse, the catalytic concentrations of seventeen enzymes and the protein and albumin concentrations in plasma were determined. The corresponding data for man were taken from the literature. The experimental conditions were selected so as to minimize the influence on catalytic activity, variance and statistical distribution pattern of such factors as handling procedures, the choice of blood drawing technique, the choice of anaesthetic agent for distinct species and the preparation procedure of plasma from the blood specimen.

Kinetic of adjustment of enzyme catalytic concentrations in the extracellular space of the man, the dog and the rat. Approach to a quantitative diagnostic enzymology, V. Communication.

The high degree of constancy of enzyme catalytic activity in the plasma of a given individual is regulated by a complex system of flux equilibria consisting of eight basic processes. Some of these processes are of primarily theoretic importance. Enzymes from all tissues of the body, including the liver, are released via a continuous physiological process into the interstitial space and get into the intravascular space by way of lymphatic transport.