Publications by authors named "I Toplak"

Article Synopsis
  • The study investigated honeybee viruses in both clinically affected and healthy honeybee colonies using advanced detection methods.
  • A total of 22 complete genome sequences of various honeybee viruses were identified, including strains like ABPV, BQCV, and DWV, among others.
  • This research marks the first complete genomic identification of several viruses in Slovenia, enhancing our understanding of honeybee virus diversity in the region.
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Honeybee diseases are one of the most significant and most common causes of honeybee colonies' weakness and death. An early diagnosis of subclinical infections is necessary to implement precautionary and control measures. Sampling debris from hive bottom boards is simple, non-invasive, and cheap.

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The Carniolan honey bee (Apis mellifera carnica) plays an essential role in crop pollination, environment diversity, and the production of honey bee products. However, the health of individual honey bees and their colonies is under pressure due to multiple stressors, including viruses as a significant threat to bees. Monitoring various virus infections could be a crucial selection tool during queen rearing.

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Lyssaviruses are the causative agents of rabies, a zoonotic, fatal disease that is thought to be ancestral to bats. In the last decade, the detection of bat associated lyssaviruses is increasing also in Europe. Within a retrospective bat associated lyssavirus surveillance study a total of 225 dead bats of 21 bat species were collected in Slovenia between 2012 and 2019 and tested by specific real-time RT-PCR method.

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Article Synopsis
  • - This study analyzes African swine fever virus (ASFV) variants in Serbia from 2019 to 2022, marking the first detailed report of its molecular characteristics since the disease was first detected in July 2019 in wild boar and domestic swine.
  • - Researchers examined 95 ASFV-positive samples from 12 infected districts, identifying multiple strains of genotype II, mainly indicated by variations in the intergenic region (IGR) between specific genes.
  • - Findings reveal three distinct clades within the CVR I group based on the B602L gene, with the majority of samples (98.4%) falling into IGR II, emphasizing the need for more research to fully understand ASFV's spread and evolution in
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