Publications by authors named "I T W Harley"
Article Synopsis
- * Plunge-freezing samples at higher temperatures presents challenges such as condensation and maintaining stable temperatures, which can affect results.
- * The paper proposes solutions to reduce condensation and temperature fluctuations by preheating equipment and controlling humidity, with successful applications shown using poly(N-isopropylacrylamide) microgels that react to temperature changes.
Chaperone-Derived Copper(I)-Binding Peptide Nanofibers Disrupt Copper Homeostasis in Cancer Cells.
Angew Chem Int Ed Engl
December 2024
Article Synopsis
In contrast to biological cell membranes, it is still a major challenge for synthetic membranes to efficiently separate ions and small molecules due to their similar sizes in the sub-nanometer range. Inspired by biological ion channels with their unique channel wall chemistry that facilitates ion sieving by ion-channel interactions, the first free-standing, ultrathin (10-17 nm) nanomembranes composed entirely of polydopamine (PDA) are reported here as ion and molecular sieves. These nanomembranes are obtained via an easily scalable electropolymerization strategy and provide nanochannels with various amine and phenolic hydroxyl groups that offer a favorable chemical environment for ion-channel electrostatic and hydrogen bond interactions.
View Article and Find Full Text PDFThe complex dynamics and transience of assembly pathways in living systems complicate the understanding of these molecular to nanoscale processes. Current technologies are unable to track the molecular events leading to the onset of assembly, where real-time information is imperative to correlate their rich biology. Using a chemically designed pro-assembling molecule, we map its transformation into nanofibers and their fusion with endosomes to form hollow fiber clusters.
View Article and Find Full Text PDFArticle Synopsis
- Systemic lupus erythematosus (SLE) and myasthenia gravis (MG) are autoimmune diseases with suggested associations, potentially increasing SLE risk following thymectomy for MG.
- A study analyzed large databases, including IBM Watson Health Explorys and the Million Veteran Program, revealing that individuals with MG were over 10 times more likely to develop SLE compared to those without MG.
- The research confirmed a strong association between SLE and MG, particularly among younger, African American women, and highlighted that MG patients who underwent thymectomy were at an even higher risk for SLE.