[Appreciative calamity investigation].

Calamity investigations often lead to recommendations that are difficult to implement and that are of little relevance, because the analysis is carried out according to a cause and effect system. The complexity of care provision requires a different method that respects the complexity of day-to-day care and is able to assess it correctly. Research according to the Safety-II philosophy clearly shows the variable interactions and dependencies present in daily work.

Preparation of leukodepleted platelet concentrates from pooled buffy coats: prestorage filtration with Autostop BC.

Background And Objectives: Our requirements for leukocyte-depleted platelet concentrates (LD-PC) for an adult patient are: platelets >240x10(9), leukocytes <5x10(6), volume of 150-400 ml; and at the end of storage a pH between 6.8 and 7.4 and presence of the swirling effect.

Leukocyte filtration mechanisms. Factors influencing the removal of infectious agents from red cell concentrates.

The purpose of the present overview was to determine the factors influencing the removal of infectious agents from red cell concentrates by filtration. In general, the efficacy of the filtration method depends on the physical as well as the functional properties of blood cells. These properties are highly influenced by the changes exerted on the blood cells during blood collection, processing and storage and the filtration method itself.

In vitro evaluation of platelet concentrates, prepared from pooled buffy coats, stored for 8 days after filtration.

Background: Posttransfusion complications can be prevented by pretransfusion removal of donor white cells from platelet concentrate. The filtration used for this removal seems to have little effect on platelet function and activation, but more information is needed on its effect on function during subsequent long-term storage of concentrate.

Study Design And Methods: The effect of prestorage filtration of buffy coat-prepared platelet concentrates (PCs) on platelet function, metabolism, and activation was investigated.