Peroxidase-labeled primary antibody method for detection of pestivirus contamination in cell cultures.

Contaminating bovine viral diarrhea (BVD) virus in cell cultures and in fetal bovine serum (FBS) is a well recognized problem. This study describes a direct peroxidase (DP) labeled primary antibody method for detection of pestivirus antigens in cell culture that is simple and reliable. Using this method, most bovine and porcine cell cultures, a cat lung, four mosquito and two monkey cell cultures were found contaminated with BVD virus.

Avidin-biotin latex agglutination assay for detection of antibodies to viral antigens.

A new method for attaching antigens to latex by an avidin-biotin technique is described. The procedure permits control of the amount of antigen attached to the latex and eliminates the need for highly purified antigens and destructive bridging chemicals. The avidin-biotin latex agglutination assay is a simple, rapid test well suited to detection of viral antibody.

Capillary enzyme immunoassay for rapid detection of herpes simplex virus in clinical specimens.

Capillary enzyme immunosorbent assay (CapELISA) is a modification of the standard enzyme immunosorbent assay which permits rapid detection of viral antigens in clinical specimens. The capillary tube format provides a very large reactive surface relative to the sample size. The close proximity of antigen to antibody in the tube optimizes the reaction, resulting in increased sensitivity and shorter incubation requirements.

Comparison of enzyme-linked immunosorbent assay with enzyme-linked fluorescence assay with automated readers for detection of rubella virus antibody and herpes simplex virus.

The enzyme-linked immunosorbent assay (ELISA) was compared with the enzyme-linked fluorescence assay (ELFA) for the detection of rubella antibody and herpes simplex virus antigen. Test parameters, specimens, antigen or antibody, and conjugates for the two types of assays were identical except that p-nitrophenyl phosphate was used as the substrate for the ELISA and 4-methylumbelliferyl phosphate was used as the substrate for ELFA. Automated readers were used for both assays.

Polycarbonate-coated microsticks as solid-phase carriers in an enzyme-linked immunosorbent assay for rubella antibody.

We evaluated the use of microsticks as solid-phase carriers in an enzyme-linked immunosorbent assay for rubella antibody. The microstick enzyme-linked immunosorbent assay was found to be equal in sensitivity to plate and disk enzyme-linked immunosorbent assays and presumably more sensitive than hemagglutination and immunofluorescence assays. The microstick as a solid-phase carrier offers advantages over both plate and bead carriers.