Publications by authors named "I Sadovskaya"
Article Synopsis
- - The cwps locus in Lactococcus lactis and Lactococcus cremoris encodes the machinery necessary for forming cell wall polysaccharides, which consist of a neutral rhamnan and various side chain oligosaccharides or polysaccharides.
- - The study identifies three new subtypes of C genotype CWPS and utilizes 2D NMR to analyze the unique structures of CWPS from four isolates, revealing distinct rhamnan and polysaccharide components.
- - Findings emphasize the structural diversity of lactococcal CWPS, suggesting that understanding these variations is crucial for improving the robustness of strains used in the fermented food industry against bacteriophages.
Lactococcus cremoris and Lactococcus lactis are among the most extensively exploited species of lactic acid bacteria in dairy fermentations. The cell wall of lactococci, like other Gram-positive bacteria, possesses a thick peptidoglycan layer, which may incorporate cell wall polysaccharides (CWPS), wall teichoic acids (WTA), and/or lipoteichoic acids (LTA). In this study, we report the isolation, purification and structural analysis of the carbohydrate moieties of glycolipids (GL) and LTA of the L.
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- The rhamnose-glucose polysaccharide (Rgp) from Streptococcus thermophilus is crucial for its cell wall and has been linked to genetic diversity across different strains, with two new genotypes identified in this study.
- Researchers analyzed 78 S. thermophilus genomes, revealing that the genetic makeup influences the structure of Rgp, which plays a key role in the bacteria's interactions with bacteriophages.
- A multiplex PCR system was developed to classify these strains based on their unique genetic content related to Rgp biosynthesis, which is significant for dairy fermentations where S. thermophilus is commonly used and is susceptible to phage attacks.
The first step in phage infection is the recognition of, and adsorption to, a receptor located on the host cell surface. This reversible host adsorption step is commonly followed by an irreversible event, which involves phage DNA delivery or release into the bacterial cytoplasm. The molecular components that trigger this latter event are unknown for most phages of Gram-positive bacteria.
View Article and Find Full Text PDFFour acteriophage-nsensitive utants (BIMs) of the dairy starter bacterium Streptococcus thermophilus UCCSt50 were isolated following challenge with Brussowvirus SW13. The BIMs displayed an altered sedimentation phenotype. Whole-genome sequencing and comparative genomic analysis of the BIMs uncovered mutations within a family 2 glycosyltransferase-encoding gene (06955) located within the variable region of the cell wall-associated hamnose-lucose olymer (Rgp) biosynthesis locus (designated the gene cluster here).
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