Binding of monoclonal anti-myelin-associated glycoprotein antibodies to human foetal peripheral neurons in culture.

Dorsal root ganglion cells, obtained from 8-10-week human foetuses, were isolated by enzymatic procedure and grown on poly-L-lysine-coated coverslips. Most of the cultured cells showed the ultrastructural and immunological features of normal peripheral neurons. By immunocytochemistry neurons reacted with IgM antibodies with specificity for myelin-associated glycoprotein (MAG) from patients affected with IgM k gammopathy and peripheral neuropathy.

Adenine nucleotides and some related enzyme activities (adenylate kinase and phosphoglycerate kinase) in normal and abnormal human semen.

Adenine nucleotides (ATP and ADP) and enzyme activities of Adenylate Kinase (AK) and Phosphoglycerate Kinase (PGK) were assayed on semen samples from normal and infertile men. In accordance with the values obtained, samples could be classified into two groups: one group with levels of either adenine nucleotides and enzymes assumed as normal; a second group with high levels of all the parameters. The latter group comprised the samples with low sperm density (less than 40 million/ml).