siRNA-Mediated Silencing Inhibited the Inflammatory Phenotype during Acute Lung Injury.

Acute lung injury is a complex cascade process that develops in response to various damaging factors, which can lead to acute respiratory distress syndrome. Within this study, based on bioinformatics reanalysis of available full-transcriptome data of acute lung injury induced in mice and humans by various factors, we selected a set of genes that could serve as good targets for suppressing inflammation in the lung tissue, evaluated their expression in the cells of different origins during LPS-induced inflammation, and chose the tissue inhibitor of metalloproteinase as a promising target for suppressing inflammation. We designed an effective chemically modified anti-TIMP1 siRNA and showed that silencing correlates with a decrease in the pro-inflammatory cytokine IL6 secretion in cultured macrophage cells and reduces the severity of LPS-induced acute lung injury in a mouse model.

[Group preventive consultation of the population concerning nutrition. Experience of School of the balanced nutrition founded on the basis of the Health center].

The development of the program of group preventive consultation of visitors of the centers of health concerning nutrition and assessment of its efficiency was the purpose of the work. The analysis of the results of inspection of 2569 visitors of the Health сenter at the age of 18–78 years and randomized, open, cross research of 242 women (27–72 years old) who passed group preventive consultation in the Center of health at «School of a balanced nutrition » were carried out. Anthropometrical data and the actual nutrition with use of the computer program «Analysis of the Person Nutrition» were studied.