[Biological, morphological and immunologic characteristics of cells transformed in vitro by bovine adenovirus type 3].

Studies of cell lines obtained as a result of in vitro transformation by bovine adenovirus type 3 at low multiplicities of infection: BHK-21/13-780 and BHK-21/13-782 (8 X 10(-5) TCD50/cell) and hamster embryo tissue HET-1 and HET-2 (2 X 10(-3) TCD50/cell) showed them to possess some oncologic characteristics typical of spontaneous neoplasia such as low degree of differentiation, high rate of cell growth, reduced adhesiveness, high tumorigenicity. In hamster embryo tissue cells (HET) transformed in vitro virus-specific transplantation antigen and S-antigen were found. In transformed cells of the initial BHK-21/13S culture possessing the capacity of transforming spontaneously and under the effect of oncogenic viruses no transplantation antigen coded for by bovine adenovirus type 3 were detected.

[The condition of mitochondria in experimental viral carcinogenesis].

A parallel histoenzymatic and electron-microscopy study of the state of mitochondria in experimental viral cancerogenesis induced by adenovirus of monkeys SA7(C8) and virus of Rous sarcoma in soft tissues of hamster was carried out. It was shown that the earliest changes in the cell in the process of its tumorous transformation were those in mitochondria. The character of deposits of diformazin at an early stage of viral cancerogenesis corresponded to the trend to the linear arrangement of mitochondria and to initial phenomena of their swelling.