Lactate dehydrogenase isoenzyme distribution and patterns in chicken organs.

Unlike most mammals, chicken lactate dehydrogenase isoenzymes cannot be separated using the 'Titan-Gel' electrophoresis. However, using isoelectric focusing at a pH range of 3.0 to 9.

Antioxidant and detoxifying enzymes in the liver and kidney of pheasants after intoxication by herbicides MCPA and ANITEN I.

The activity of antioxidant and detoxifying enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase, glutathione-S-transferase (GST), the contents of thiobarbituric acid reactive substances, and the superoxide dismutase and glutathione-S-transferase isoenzyme patterns, were determined in the liver and kidney of pheasants after acute intoxication by herbicides MCPA and ANITEN I. In the liver, the activity of antioxidant enzymes was significantly decreased in the group given ANITEN I. New superoxide dismutase isoforms (pI 6.

Antioxidant and detoxifying fish enzymes as biomarkers of river pollution.

The activity of several antioxidant and detoxifying enzymes, superoxide dismutase SOD, GSH peroxidase GSHPx, GSSG reductase GSR and GSH S transferase GST, the contents of thiobarbituric acid reactive substances TBARS, and the SOD and GST isoenzyme patterns were studied in the livers of chubs Leuciscus cephalus from reference river areas and polluted urban sites. Livers of polluted fish contained higher concentrations of transition metals, especially copper and iron. Total GSHPx activity was 1.

Changes in GST-isoenzyme pattern of some organs of sheep exposed to different levels of pollution.

GST isonzyme patterns were studied in the cytosolic fraction of liver, kidney and lung of sheep exposed to industrial metal pollutants and compared with those of control animals. The methodology included the determination of enzymatic activities with several subunit-specific substrates (DCNB, NPB, EPNP and EA) and Western blotting using antibodies to specific rat GST subunits 1, 8 (alpha class), 3 (mu class) and 7 (pi class). In liver and lung, crossed reactivities with subunits 1 and 3 were absent in the controls but were present in exposed animals.

Effects of iron and vitamin E administration on some immunological parameters in pigs.

The effects of intramuscular administration of iron and vitamin E on phagocytic functions of leucocytes and on T-lymphocyte percentages were studied. Twelve pigs with an average body weight (BW) of 40 kg were divided into four treatment groups: +E (20 mg tocopheryl acetate per kg BW); +Fe (10 mg iron dextran per kg BW); +E/+Fe (20 mg tocopheryl acetate + 10 mg iron dextran per kg BW), C (control). Blood samples were collected from the sinus ophthalmicus prior to administration the preparations, and then at 12, 24, 48, 72 hours, and 7 days after administration.