Cytotoxicity of cadmium, selenium, zinc and copper to mouse myeloma sp2/0 cells as measured by the MTT assay.

As part of our general programme to test the toxicity in vitro of heavy metals and, in particular, the interactive cytotoxicity of combinations of these metals, the MTT (tetrazolium) assay was used to test the cytotoxicities of cadmium [Cd (O2CCH3)2], selenium (Na2SeO3), zinc (ZnSO4) and copper (CuSO4) with an established mouse myeloma cell line (Sp2/0). The influence on the cytotoxicity of cadmium of added subtoxic concentrations of the other three metals was also studied. The IC50 values (50% inhibitory concentrations) to the Sp2/0 cells were as follows: Cd - 10μM, Se - 1μM, Zn - 10μM and Cu - 100μM.

Toxicity of Mercury to Hybridoma TA7 Cells.

Environmental mercury and mercury compound contamination has increased dramatically since the industrial revolution. This paper describes the toxic effects of mercury on a culture of hybridoma TA7 cells, which produce antibodies against the A-subunit of viskumin. Cells were cultivated on 96-well flat-bottomed plates with RPMI-1640 medium supplemented with 10% fetal calf serum at 37°C in 5% CO2/95% air.

[Immunological monitoring and correction of the immune disorders in allergic diseases in poultry growers].

It was established that allergic diseases of poultry farmers were characterized by the shifts in immunoregulation expressed in terms of the decrease of the number of T-lymphocyte-suppressors, precursors of effectory and immunoregulatory T-lymphocytes, reduction of functional actifity of T- and B-cells along with an increase in the content of B-lymphocytes with antibody-dependent killers' sensitization and suppression of nonspecific protective factors. The results suggested that the use of immunomodulatory drugs helped to improve clinicoimmunologic indicators.