Publications by authors named "I Piuz"
Introduction: Rhinovirus (RV) infections constitute one of the main triggers of asthma exacerbations and an important burden in pediatric yard. However, the mechanisms underlying this association remain poorly understood.
Methods: In the present study, we compared infections of reconstituted airway epithelia originating from asthmatic versus healthy donors with representative strains of RV-A major group and minor groups, RV-C, RV-B, and the respiratory enterovirus EV-D68.
Article Synopsis
- Enteroviruses (EVs), highly diverse viruses, can cause various symptoms; EV-D68 (respiratory) and EV-D94 (enteric) illustrate this diversity despite being in the same species.
- The study utilized 3D tissue culture models to show how temperature affects tissue tropism and identified key differences in how intestinal and respiratory tissues respond to these viruses.
- Transcriptomic analysis revealed that EV-D68 activates antiviral pathways while EV-D94 minimizes immune responses, highlighting distinct strategies in their interactions with host tissues.
Enterovirus 71 (EV71) causes hand, foot and mouth disease, a mild and self-limited illness that is sometimes associated with severe neurological complications. EV71 neurotropic determinants remain ill-defined to date. We previously identified a mutation in the VP1 capsid protein (L97R) that was acquired over the course of a disseminated infection in an immunocompromised host.
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- Enteric and respiratory enteroviruses (EVs) share genetic similarities but exhibit significant differences in their biophysical properties, which affect where they replicate in the body and how virulent they are.
- Researchers created chimeric viruses combining elements from EV-D68 (a respiratory virus) and EV-D94 (an enteric virus) to study these differences, discovering that capsid proteins influence factors like acid sensitivity and tissue tropism.
- Investigations revealed that the capsid structure affects temperature adaptation and immune response interaction, providing insights that might be useful for developing vaccines or antiviral treatments for these common pathogens.
Background: The leading cause of acute illnesses, respiratory viruses, typically cause self-limited diseases, although severe complications can occur in fragile patients. Rhinoviruses (RVs), respiratory enteroviruses (EVs), influenza virus, respiratory syncytial viruses (RSVs), and coronaviruses are highly prevalent respiratory pathogens, but because of the lack of reliable animal models, their differential pathogenesis remains poorly characterized.
Objective: We sought to compare infections by respiratory viruses isolated from clinical specimens using reconstituted human airway epithelia.