FIT-PNAs as RNA-Sensing Probes for Drug-Resistant .

Detecting RNA at single-nucleotide resolution is a formidable task. is the deadliest form of malaria in humans and has shown to gain resistance to essentially all antimalarial drugs including artemisinin and chloroquine. Some of these drug resistances are associated with single-nucleotide polymorphisms (SNPs).

High-speed temporal and spatial beam-shaping combining active and passive elements.

Temporal and spatial shaping of laser beams is common in laser micromachining applications to improve quality and throughput. However, dynamic beam shaping (DBS) of ultrashort, high-power pulses at rates of hundreds of kHz has been challenging. Achieving this allows for full synchronization of the beam shape with high repetition rates, high-power lasers with zero delay time.

DNA methylation patterns expose variations in enhancer-chromatin modifications during embryonic stem cell differentiation.

In mammals, cellular identity is defined through strict regulation of chromatin modifications and DNA methylation that control gene expression. Methylation of cytosines at CpG sites in the genome is mainly associated with suppression; however, the reason for enhancer-specific methylation is not fully understood. We used sequential ChIP-bisulfite-sequencing for H3K4me1 and H3K27ac histone marks.

Predictive Model for the Sequence-Dependent Fluorogenic Response of Forced-Intercalation Peptide Nucleic Acid.

Unlabelled: The forced-intercalation peptide nucleic acid (FIT-PNA) concept, introduced by Seitz and co-workers, is based on replacing a nucleobase of the PNA sequence with a cyanine dye (such as thiazole orange). The cyanine dye is thus a surrogate base that is forced to intercalate in the duplex (e.g.

Validation of the QOLIBRI - Quality of Life after Brain Injury questionnaire in patients after TBI in Israel.

Background: The QOLIBRI - Quality of Life after Brain Injury questionnaire was developed by the QOLIBRI Task Force (QTF). Our goal was to investigate the applicability, validity and reliability of the QOLIBRI in Israel.

Methods: Validation of the Hebrew questionnaire was performed after it had been administered to 128 adults with traumatic brain injury (TBI), who were between 3 months' and 15 years' post-discharge from rehabilitation.