Publications by authors named "I Papantoniou"
Self-assembled cartilaginous microtissues provide a promising means of repairing challenging skeletal defects and connective tissues. However, despite their considerable promise in tissue engineering, the mechanical response of these engineered microtissues is not well understood. Here we examine the mechanical and viscoelastic response of progenitor cell aggregates formed from human primary periosteal cells and the resulting cartilaginous microtissues under large deformations as might be encountered in vivo.
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- Research on multicellular spheroids like microtissues and organoids highlights their potential in tissue engineering due to enhanced interactions in 3D cell structures.
- A new laser-assisted bioprinting technique has been developed that successfully transfers these spheroids to create larger tissue structures, demonstrating high cell viability and differentiation capability.
- The method includes an innovative approach called LIPMO, allowing for the bioprinting of larger spheroids (up to 300 micrometers) while utilizing automated image analysis for precise selection and placement of multiple spheroids in a single printing sequence.
Automated technologies are attractive for enhancing the robust manufacturing of tissue-engineered products for clinical translation. In this work, we present an automation strategy using a robotics platform for media changes, and imaging of cartilaginous microtissues cultured in static microwell platforms. We use an automated image analysis pipeline to extract microtissue displacements and morphological features as noninvasive quality attributes.
View Article and Find Full Text PDFBackground: Clinical characteristics and outcomes of patients with transthyretin amyloidosis cardiomyopathy (ATTR-CM) vary by region, necessitating the acquisition of country-specific evidence for proper management.
Methods: This is an observational study including sequential patients presenting in the Amyloidosis Reference Center of Greece, from 01/2014 to 12/2022. ATTR-CM was diagnosed by positive scintigraphy and exclusion of light-chain amyloidosis or positive biopsy typing.
Cartilage microtissues are promising tissue modules for bottom up biofabrication of implants leading to bone defect regeneration. Hitherto, most of the protocols for the development of these cartilaginous microtissues have been carried out in static setups, however, for achieving higher scales, dynamic process needs to be investigated. In the present study, we explored the impact of suspension culture on the cartilage microtissues in a novel stirred microbioreactor system.
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