Intrarenal distribution of ampicillin in the healthy and the infected rat kidney: a comparison of methods.

The ampicillin concentration in the cortex of the normal and the infected rat kidney was determined by means of three different methods: tissue homogenate analysis, renal lymph analysis, and autoradiography. Both tissue homogenate analysis and autoradiography revealed that the cortical ampicillin concentration was about five times as high as the serum concentration. Renal lymph analysis disclosed that the ampicillin concentration in this fluid amounted to about 93% of the serum concentration.

Ampicillin susceptibility and ampicillin-induced killing rate of Escherichia coli.

The killing rate induced by ampicillin was determined in 20 strains of Escherichia coli. The apparent generation rate constant for each E. coli showed a characteristic concentration-dependent course.