Study of changes in diacylglycerol content on nerve excitation.

Rhythmic excitation of a rabbit myelin nerve increased diacylglycerol (DAG) content from 1.53 to 2.17 microg/mg lipids.

[Activity of phosphoinositide-specific phospholipase C in rabbit nerves at rest and during stimulation].

The dependence of rabbit sciatic nerve PtdIns-specific phospholipase C activity Ca2+ and pH has been studied. The enzyme was found to hydrolyze PtdInsP2 in a Ca(2+)-dependent manner. The maximal activity was detected at 1mM CaCl2 for the cytosolic enzyme and at 0.