Characterization of kappa opioid receptors in the rabbit ear artery.

Quantitative characterization of the kappa opioid receptor in the rabbit ear artery was carried out using three kappa-selective agonist compounds, dynorphin-(1-13), U-69593 and ethylketocyclazocine. Kinetic analysis was performed using the antagonist, MR 2266. Two other in vitro preparations were studied for comparison: the mouse was deferens and rabbit was deferens.

Characterization of rabbit ear artery opioid receptors using a delta-selective agonist and antagonist.

In order to characterize pre-junctional delta opioid receptors in the rabbit ear artery, the delta-selective agonist [D-Pen2,D-Pen5]enkephalin (DPDPE), and delta-selective antagonist, ICI 174,864, were used. The selectivity of these compounds for the delta receptor was confirmed using a standard opioid sensitive preparation, the mouse was deferens. Relative potencies of a series of opioid agonists were similar in the rabbit ear artery and the mouse vas deferens.

Effect of two synthetic alpha-gliadin peptides on lymphocytes in celiac disease: identification of a novel class of opioid receptors.

Two synthetic peptides containing residues 43-47 and 43-49 of alpha-gliadin were tested for inhibition of leukocyte migration in 47 patients with celiac disease. In nineteen patients, all on a normal diet, leukocyte migration was inhibited by the peptides and naloxone blocked this effect. In twenty-eight patients (24 of whom were on strict gluten-free diet) leukocyte migration was not affected by the peptides.

Delta and kappa opioid receptors in the rabbit ear artery.

Delta and kappa opioid receptors have been characterized in the central ear artery of the rabbit. The delta selective D-Pen2,D-Pen5-enkephalin and the highly selective delta antagonist ICI 174864 have been used for the delta opioid receptors. Ke values were determined and compared to that of the electrically stimulated mouse vas deferens preparation.