Trans-synaptic regulation of calmodulin gene expression after experimentally induced orofacial inflammation and subsequent corticosteroid treatment in the principal sensory and motor trigeminal nuclei of the rat.

The cutaneous and mucosal surfaces in the infraorbital region around the whisker pad are innervated by the maxillary division of the afferent fibers of the trigeminal nerve, while certain ganglion cells project to the principal sensory trigeminal nucleus (Pr5). In turn, some of the neurons in the Pr5 project to the motor trigeminal nucleus (Mo5), whose neurons do not innervate the infraorbital skin. We analyzed the calmodulin (CaM) gene expression in these nuclei after dithranol-induced inflammation and subsequent treatment with corticosteroid in the infraorbital skin.

Dithranol abolishes UCH-L1 immunoreactivity in the nerve fibers of the rat orofacial skin.

Dithranol has been used to treat psoriasis for decades. Although its beneficial effect may involve the induction of cutaneous inflammation, and inflammation often leads to damages in nerve fibers, these alterations are not well documented. Therefore, we investigated the effects of dithranol on the immunohistochemical characteristics of the cutaneous nerve fibers in the rat skin.

Differential calmodulin gene expression in the nuclei of the rat midbrain-brain stem region.

We investigated the expression patterns of the three calmodulin (CaM) genes, using in situ hybridization techniques, to detect gene-specific [(35)S]- and digoxigenin-labeled cRNA probes complementary to the multiple CaM mRNAs in the nuclei of the midbrain-brain stem region of the adult rat. The distinct CaM genes were widely expressed throughout this region with moderate intensities. In spite of the similar general pattern, significant differences in the distributions of the multiple CaM mRNA species were found in certain areas.

[Fertility after removal of intra-uterine device (author's transl)].

A survey of cases was made in the context of an outpatient family planning scheme. Intra-uterine devices were removed from 874 women who had expressed interest in another pregnancy. The group of probands, in that context, comprised 701 women.

[Laparoscopy and hysterosalpingography in sterility examination (author's transl)].

Hysterosalpingographic and laparoscopic findings were recorded from 90 patients and compared. They were identical in 71 per cent of all cases, but in some of the cases additional findings proved recordable from laparoscopy. The point is made by the authors that laparoscopy should in any case be preceded by hysterosalpingography, since the latter is a comparatively uninvolved method which even may have therapeutic effects.