Lipid studies in primary gout.

Serum total cholesterol, HDL cholesterol, LDL cholesterol, and triglyceride were measured in three groups of male patients with primary gout. The groups were defined by the presence of 0, 1, or more than 1 coexisting medical conditions or cardiac risk factors (coronary heart disease, hypertension, diabetes, proteinuria, overweight). Mean values of total, LDL, and HDL cholesterol were lower in patients with one or more associated conditions than in those with none.

Reactivity of sized thermal aggregates of immunoglobulin G with IgM rheumatoid factor.

Solutions of purified pooled human IgG heated for varying times and temperatures between 55 degrees and 65 degrees yielded soluble turbid non-immune polydispersed aggregate mixtures. Gel filtration of IgG aggregate mixtures on calibrated Biorad A-15, A-50 or A-150 columns resulted in continuous size distributions from dimers to particles with mol. wts.

Size fractionation of thermal aggregates of immunoglobulin G.

Purified pooled human immunoglobulin G (IgG) in solution, when extensively heated at high temperatures or for long periods, irreversibly aggregates and insoluble precipitates result. However, when IgG solutions are heated in the temperature range 55-65 degrees C for more limited time periods, soluble turbid polydispersed aggregate mixtures are obtained. Gel filtration of such aggregate mixtures on calibrated Bio-Rad A-150m columns demonstrates a continuous size distribution from dimers to aggregates as large as 4 X 10(7) Da (200-mers) with no particular size predominant.