S19-mRNA expression in squamous cell carcinomas of the upper aerodigestive tract.

Background: The differential display method showed altered expression of ribosomal protein S19 gene in human head and neck squamous cell carcinoma (HNSCC) cell lines.

Materials And Methods: To verify these results, RT-PCR analysis was carried out in 18 HNSCC and 17 benign epithelial cell lines as well as 30 HNSCC and 8 reference tissue samples. In the HNSCC cells S19 mRNA expression was significantly reduced as compared to benign epithelial cells.

Reduction of LOX- and LOXL2-mRNA expression in head and neck squamous cell carcinomas.

Background: Besides other molecular functions, the lysyl oxidase gene (LOX) has been assumed and shown to have a tumor suppressive function in vitro and in vivo. The cancer-related functions of both LOX and LOXL2 have not yet been investigated in squamous cell carcinoma of the head and neck (HNSCC).

Materials And Methods: We examined the mRNA levels of LOX and LOXL2 in ten malignantly transformed cell lines and sixteen malignant tissue samples of different graded and staged HNSCC by RT-PCR.

The role of matrix metalloproteinases in squamous cell carcinomas of the head and neck.

The ability of tumors to infiltrate the surrounding tissue is one of the major characteristics of a malignancy. This process is based on the tumors ability to destroy the extracellular matrix (ECM) including the basement membrane (BM). Several previous studies identified matrix metalloproteinases (MMPs), a family of zinc-dependent endopeptidases to be key players in this process.

mRNA differential display of human laryngeal carcinoma cells and corresponding benign keratinocytes: cloning versus PCR-amplification.

The mRNA expression profile of mucosal keratinocyte cell lines was compared to that of squamous cell carcinoma cell lines of the head and neck (UMSCC-10A and UTSCC-19A) by the differential display technique. Before the sequencing procedure, the differentially expressed fragments can be reamplified either by PCR amplification only or by PCR amplification combined with molecular cloning. In this study, these two methods are compared.

Transcriptional repression of the human galactocerebrosidase gene in squamous cell carcinomas of the larynx.

Alterations of gene expression in squamous cell carcinoma (SCC) cell lines derived from the larynx and keratinocytes derived from adjacent normal mucosa of the larynx have been studied using the mRNA differential display technique. Lane-to-lane comparison of reverse transcribed mRNA showed a strong repression of a 148 bp fragment in SCC cells. The fragment was reamplified and cloned.