[Genetic transformation of the moss Ceratodon purpureus by novel polycationic carriers of DNA].

There is a big progress in application of genetic engineering for improving the biological properties of different organisms. Viral and non-viral carriers are used for delivery of genetic material into target cells. Nanoscale polymeric materials of natural and synthetic origin are the most promising gene delivery agents.

[cDNA library construction from panicle meristem of finger millet].

The protocol for production of full-size cDNA using SuperScript Full-Length cDNA Library Construction Kit II (Invitrogen) was tested and high quality cDNA library from meristematic tissue of finger millet panicle (Eleusine coracana (L.) Gaertn) was created. The titer of obtained cDNA library comprised 3.

[Mapping a new secalin locus on the rye iRS arm].

A gene designated Sec-N encoding secalin was mapped in the introgressive winter common wheat line Hostianum 273/97 (H273) with the wheat-rye substitution (1B)1R from the octoploid triticale AD825. F2 seeds from crossing the line H273 with the line Hostianum 242/97-2 carrying the wheat-rye 1BL/1RS translocation were analysed. The studied component on the SDS-electrophoregram of total proteins was revealed to be a monomeric secalin which is encoded by the gene at the new locus Sec-N located distally with respect to the Sec-1 locus at a distance of 21.

[Obtaining the transgenic lines of finger millet Eleusine coracana (L.) Gaertn. With dinitroaniline resistance].

The current data is dedicated to the study of bioballistic and Agrobacterium-mediated transformation of finger millet with the constructs carrying the mutant alpha-tubulin gene (TUAm 1), isolated from R-biotype goosegrass (Eleusine indica L.), for the decision of problem of dinitroaniline-resistance. It was found that 10 microM of trifluralin is optimal for the selection of transgene plants of finger millet.

[Creation of transgenic sugar beet lines expressing insect pest resistance genes cry1C and cry2A].

Impact of insect pests makes a significant limitation of the sugar beet crop yield. Integration of cry-genes of Bacillus thuringiensis into plant genome is one of the promising strategies to ensure plant resistance. The aim of this work was to obtain sugar beet lines (based on the MM 1/2 line) transformed with cry2A and cry1Cgenes.