Publications by authors named "I Neganova"
Article Synopsis
- Human pluripotent stem cells (hPSCs) can differentiate into any adult tissue, making them important for regenerative medicine and research into factors affecting their growth and characteristics.
- The study analyzed the migration patterns of three hPSC cell lines (AD3, CaSR, and H9) through bright-field imaging, focusing on their "good" and "bad" morphological phenotypes.
- Results showed that migration speed varied based on the cell line and growth environment, with notable differences in migration behavior between "good" and "bad" colonies, particularly in specific culture conditions.
Article Synopsis
- - The study focuses on correcting mutations in the CASR gene, which is crucial for regulating calcium levels in the body, specifically targeting mutations linked to a severe condition called neonatal primary hyperparathyroidism.
- - Researchers generated a CRISPR/Cas9 edited human induced pluripotent stem cell (hiPSC) line that successfully restored one of the mutations while preserving another, showing normal characteristics such as cell structure and growth potential.
- - The newly created hiPSC line will be used as a valuable resource for understanding calcium regulation disorders and exploring personalized medicine approaches to treat these conditions in the future.
Mesenchymal stem cells lose the senescent phenotype under 3D cultivation.
Stem Cell Res Ther
December 2023
Background: Three-dimensional (3D) cell culture is widely used in various fields of cell biology. In comparison to conventional two-dimensional (2D) cell culture, 3D cell culture facilitates a more accurate replication of the in vivo microenvironment, which is essential for obtaining more relevant results. The application of 3D cell culture techniques in regenerative medicine, particularly in mesenchymal stem cell (MSC)-based research, has been extensively studied.
View Article and Find Full Text PDFHuman pluripotent stem cells have the potential for unlimited proliferation and controlled differentiation into various somatic cells, making them a unique tool for regenerative and personalized medicine. Determining the best clone selection is a challenging problem in this field and requires new sensing instruments and methods able to automatically assess the state of a growing colony ('phenotype') and make decisions about its destiny. One possible solution for such label-free, non-invasive assessment is to make phase-contrast images and/or videos of growing stem cell colonies, process the morphological parameters ('morphological portrait', or signal), link this information to the colony phenotype, and initiate an automated protocol for the colony selection.
View Article and Find Full Text PDFAs a highly dynamic organ, bone changes during throughout a person's life. This process is referred to as 'bone remodeling' and it involves two stages - a well-balanced osteoclastic bone resorption and an osteoblastic bone formation. Under normal physiological conditions bone remodeling is highly regulated that ensures tight coupling between bone formation and resorption, and its disruption results in a bone metabolic disorder, most commonly osteoporosis.
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