Evaluation of the efficacy of granulocyte colony-stimulating factor for the treatment of experimental myocardial destruction in mice.

We studied the effects of recombinant granulocytic CSF on heart remodeling in BALB/c mice after cryodestruction. Administration of granulocytic CSF was started 1 day after cryodestruction (subcutaneously, 10 μg/kg/day, for 4 days). As early as after the first injection, leukocytosis in the peripheral blood started to develop, leukocyte count peaked on days 4-6 and returned to normal on day 14.

Comparative assessment of heart remodeling in rats after experimental coronary stenosis and cryodestruction.

Comparative analysis of myocardial changes due to heart remodeling after experimental coronary stenosis and cryodestruction in rats was performed. Similar picture of heart remodeling was observed in all animals on day 45 irrespective of the type of destruction: hypertrophy of intact myocardium of the left ventricle, formation of extensive connective tissue cicatrix, and similar structural changes in the myocardium adjacent to the damage area. The type of the damaging influence possibly plays a role at the stage of lesion formation.

Effect of transplantation of bone marrow cells on morphology of rat myocardium after cryodestruction.

We studied the effects of bone marrow cell transplantation on myocardium of the prenecrotic zone in Wistar rats. Intramyocardial cell transplantation reduced the severity of hypertrophy of myocardium and the degree of its cicatricial degeneration on day 40 after cryodestruction. The morphology of the myocardium in the prenecrotic zone depended on the type of transplanted cells.

Colony-forming cells in rat myocardium after destructive exposure and intramyocardial transplantation of bone marrow cells.

The content of colony-forming cells in myocardial cell culture from the perinecrotic zone of rat heart was evaluated on day 40 after cryodestruction. The mean cellularity after cryodestruction was 12-fold lower than in intact animals. Intramyocardial transplantation of bone marrow cells (mononuclears, mesenchymal stem cells, and mesenchymal stem cells treated with 5-azacitidine) into the perinecrotic zone increased the content of colony-forming cells.

[Nonviral gene transfer in vivo in gene therapy].

Recent findings connected with in vivo use of artificial macromolecular complexes (genosomes) for functional gene transfer and delivery are discussed in the paper. Non-viral methods are the most safe for the purpose of human gene delivery. The cationic liposomes containing cholesterol are the most suitable for this purpose, because they possess high biodegradability and stability in blood stream.