[Activation of JAK/STAT signaling pathway in S2 Drosophila melanogaster cell culture].

JAK/STAT signaling pathway plays a critical role in different ontogenesis processes of higher eukaryotes. Fruit fly drosophila is a handy model system used to study this pathway since major components of the pathway are represented by unique factors. This article describes the usage of Drosophila melanogaster S2 cells in studies of the pathway's target genes activation.

[Molecular components of JAK/STAT signaling pathway and its connection with transcription machinery].

JAK/STAT is one of the conservative signaling pathways in higher eukaryotes which plays a critical role in different ontogenesis processes. This article gives a review of the pathway structure at the molecular level, mainly in a model organism Drosophila. There are data about relationship of the signaling pathway and transcription machinery of higher eukaryotes.

[SAYP interacts with DHR3 nuclear receptor and participates in ecdysone-dependent transcription regulation].

The role of metazoan coactivator SAYP in nuclear receptor-driven gene activation in the ecdysone cascade of Drosophila is considered. SAYP interacts with DHR3 nuclear receptor and activates the corresponding genes by recruiting the BTFly (Brahma and TFIID) coactivator supercomplex. The knockdown of SAYP leads to a decrease in the level of DHR3-activated transcription.

[Functioning of macromolecular complexes at successive stages of gene expression as self-coordinated molecular machines].

The results of systemic investigations devoted to the role of the proteins SAYP and ENY2 from Drosophila melanogaster in the regulation of different stages of gene expression are presented. The gene expression is a system of multistage processes involving the preparation of the chromatin matrix, initiation of transcription, mRNA synthesis, as well as the formation of mPNR particles, their export, and translation in the cytoplasm. At each of these stages, a great number of factors present6ed usually by protein complexes consisting of different subunits work.

[Multifunctional factor ENY2 integrates the various stages of gene expression].

The ENY2/Sus1 is a multifunctional transcription factor which couples transcription with mRNA export. It is the component of the SAGA/TFTC and TREX-2/AMEX protein complexes. Recently, we described the interaction of ENY2 with one more protein complex, THO.