Publications by authors named "I N Maslov"
The primary role of telomerase is the lengthening of telomeres. Nonetheless, emerging evidence highlights additional functions of telomerase outside of the nucleus. Specifically, its catalytic subunit, TERT (Telomerase Reverse Transcriptase), is detected in the cytosol and mitochondria.
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- Solvatochromic compounds are useful probes for biological research, specifically for tracking changes in protein structures.
- The study utilized thiol-reactive solvatochromic analogs of the GFP chromophore to monitor two proteins: recoverin and the A adenosine receptor (AAR), finding that the best dye (DyeC) showed significant fluorescence changes related to protein activation.
- The research highlights the potential of GFP-inspired dyes to effectively detect structural changes in G protein-coupled receptors (GPCRs), providing benefits like enhanced sensitivity to conformational changes and the ability to track fluorescence changes in response to different ligands.
Background: The renin-angiotensin system involves many more enzymes, receptors and biologically active peptides than originally thought. With this study, we investigated whether angiotensin-(1-5) [Ang-(1-5)], a 5-amino acid fragment of angiotensin II, has biological activity, and through which receptor it elicits effects.
Methods: The effect of Ang-(1-5) (1µM) on nitric oxide release was measured by DAF-FM staining in human aortic endothelial cells (HAEC), or Chinese Hamster Ovary (CHO) cells stably transfected with the angiotensin AT -receptor (AT R) or the receptor Mas.
Protein diffusion is a critical factor governing the functioning and organization of a cell's cytoplasm. In this study, we investigate the influence of (poly)ribosome distribution, cell aging, protein aggregation, and biomolecular condensate formation on protein mobility within the E. coli cytoplasm.
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