[Influence of low doses of ionizing radiation on tenascin expression in hybridoma cell systems].

One of the achievements of the modern radiation ecology is the preparation and application of stable eukariotic cell lines to solve various problems occurring under exposure to ionizing radiation, especially to low doses. The detection of onco-fetal protein--tenascin in different embryonic and tumor cells of humans and animals supposes the probability of appropriate gene expression in lymphoid cells, including hybridomal cells. Using the immunochemical method, the study of tenascin expression in two mouse hybridomal lines was carried out.

[Polymorphism of exon 4 in the CANP-3 gene in patients with primary myopathies].

The structures of the gene for calpain (CANP-3) and of the DMD gene were analyzed in patients with primary myopathies [limb-girdle muscular distrophy (LGMD) and Duchenne-Becker myodystrophy (DBM)] from various regions of Russia. Via amplification of DNA isolated from the peripheral blood lymphocytes of 74 patients, extended deletions were found in 18 out of 55 patients with DBM. In none of the 19 patients with LGMD, were extended deletions in the CANP-3 gene found.

[The use of low doses of prednisolone for the treatment of patients with Duchenne-Becker myodystrophy].

A special program for long-term application of low-dose prednisolone treatment in Duchenne-Becker muscular dystrophy with complex control of the patients' state was developed. Three-month cycles of prednisolone treatment alternated with three-month cycles of placebo during the year. Each patient received 0.

[A databank on Russian families with hereditary neuromuscular diseases].

The information about 5 thousands Russian families with hereditary neuromuscular disorders (HNMD) was collected by means of both different genetic epidemiological methods and authors' own observations. On the basis of this material a computer database MYODYS in Excel 5.0 format was created, which included information about 30 different signs concerning 1920 families from 70 regions of Russia.

[Expression of tenascin in lymphoid cells and changes in it upon exposure to low doses of x-rays].

The oncofetal protein tenascin was detected in some strains of lymphoid cells: murine myeloma X-63, murine hybridoma MLC-1 and K-48 and T-cell plasmacytoma MOLT-4. This effect was in agreement with the concept that tenascin serves as a marker of cell malignancy; such a concept allows one to use the marker in the studies of X irradiation impact on hybridoma clones. A non-linear correlation was found between the content of tenascin and the irradiation dosage, by using two hybridoma strains.