Publications by authors named "I N Konstantinova"
Enzymatic Transglycosylation Features in Synthesis of 8-Aza-7-Deazapurine Fleximer Nucleosides by Recombinant PNP: Synthesis and Structure Determination of Minor Products.
Biomolecules
July 2024
Article Synopsis
- Enzymatic transglycosylation of a specific fleximer base using recombinant purine nucleoside phosphorylase (PNP) resulted in the production of both expected and unexpected reaction products, including N1-pyrazole nucleosides and novel derivatives.
- The study revealed that certain minor products, like a 4-imino-pyridinium riboside and bis-ribose derivatives, were formed, while a particular expected product (4-imino-pyridinium deoxyriboside) was absent.
- Molecular modeling helped identify how the structure of the heterocyclic substrates interacts with the PNP active site, suggesting that the spatial arrangement may facilitate secondary glycosylation, leading to
1,2,4-Triazole derivatives have a wide range of biological activities. The most well-known drug that contains 1,2,4-triazole as part of its structure is the nucleoside analogue ribavirin, an antiviral drug. Finding new nucleosides based on 1,2,4-triazole is a topical task.
View Article and Find Full Text PDFThe rapid increase in the antibiotic resistance of microorganisms, capable of causing diseases in humans as destroying cultural heritage sites, is a great challenge for modern science. In this regard, it is necessary to develop fundamentally novel and highly active compounds. In this study, a series of -alkylcytidines, including 5- and 6-methylcytidine derivatives, with extended alkyl substituents, were obtained in order to develop a new generation of antibacterial and antifungal biocides based on nucleoside derivatives.
View Article and Find Full Text PDFA variety of ribo-, 2'-deoxyribo-, and 5'-norcarbocyclic derivatives of the 8-aza-7-deazahypoxanthine fleximer scaffolds were designed, synthesized, and screened for antibacterial activity. Both chemical and chemoenzymatic methods of synthesis for the 8-aza-7-deazainosine fleximers were compared. In the case of the 8-aza-7-deazahypoxanthine fleximer, the transglycosylation reaction proceeded with the formation of side products.
View Article and Find Full Text PDFTwo forms were found in the NMR spectra of N-substituted 2-chloroadenosines. The proportion of the mini-form was 11-32% of the main form. It was characterized by a separate set of signals in COSY, N-HMBC and other NMR spectra.
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