[Implementation of the national project «Healthcare»: territorial, organizational and personnel aspects].

The article examines the key aspects of the development of Russian healthcare through the prism of the implementation of the National Project «Healthcare»: improving the quality of life of Russians through the search for effective mechanisms; obtaining a social effect through the use of new state capabilities and existing potential. Currently, ensuring the availability of medical care for residents of remote areas, including through the development of preventive measures and the use of modern diagnostic tools, is of paramount importance for maintaining the population and ensuring the well-being of citizens. In conclusion, it is concluded that over the years of the implementation of regional health development projects, significant positive results have been achieved, which have allowed a positive impact on the effectiveness and quality of medical care, and improve the overall morbidity of the population.

Two-dimensional high-throughput on-cell screening of immunoglobulins against broad antigen repertoires.

Identifying high-affinity antibodies in human serum is challenging due to extremely low number of circulating B cells specific to the desired antigens. Delays caused by a lack of information on the immunogenic proteins of viral origin hamper the development of therapeutic antibodies. We propose an efficient approach allowing for enrichment of high-affinity antibodies against pathogen proteins with simultaneous epitope mapping, even in the absence of structural information about the pathogenic immunogens.

Effective bridging strategies prior to infusion with tisagenlecleucel results in high response rates and long-term remission in relapsed/refractory large B-cell lymphoma: findings from a German monocentric study.

Background: Incorporating chimeric antigen receptor (CAR)-T cell therapy into relapsed or refractory large B-cell lymphoma (rr LBCL) treatment algorithms has yielded remarkable response rates and durable remissions, yet a substantial portion of patients experience progression or relapse. Variations in outcomes across treatment centers may be attributed to different bridging strategies and remission statuses preceding CAR-T cell therapy.

Patients: Twenty-nine consecutive adult patients receiving tisagenlecleucel (tisa-cel) for rr LBCL from December 2019 to February 2023 at Jena University Hospital were analyzed.

[Mechanisms of state support for the rehabilitation of disabled people: problems and directions for their solution].

The article is devoted to the analysis of the mechanisms of state support for the rehabilitation of disabled people. Given the limitation of the functional state of people with disabilities, providing them with rehabilitation services is an important component of maintaining and preventing the deterioration of their physical condition and health. In addition, taking into account the limited opportunities for work, rehabilitation and social support measures are an important part of the material support of the disabled and an important measure to prevent poverty among them by the state.

Phage Immunoprecipitation Sequencing (PhIP-Seq) for Analyzing Antibody Epitope Repertoires Against Food Antigens.

While thousands of food and environmental allergens have been reported, conventional methods for allergy testing typically rely on measuring immunoglobulin E (IgE) binding against panels of dozens to hundreds of antigens. Beyond IgE, also the specificity of other Ig (sub-)classes such as IgG4, has gained interest because of a potential protective role toward allergy.Phage immunoprecipitation sequencing (PhIP-Seq) allows to study hundreds of thousands of rationally selected peptide antigens and to resolve binding specificities of different Ig classes.