Protocol for adeno-associated virus-mediated miRNA delivery in a rat heart failure model.

Delivering microRNA (miRNA) to treat cardiac disease is a significant challenge, and selecting an efficient delivery method to target the affected organ is critical for therapeutic success. Here, we present a protocol for delivering miRNA to the heart of rats with heart failure using adeno-associated virus (AAV9) coupled with a hydrodynamic transfusion strategy. We describe steps for inducing heart failure, echocardiography, and AAV9 application.

Concomitant telomere attrition is associated with spinal muscular atrophy in highly inbred region of North India: unraveling the thread in Kashmir region.

Spinal muscular atrophy (SMA) is a rare genetic disorder that unequivocally results in the degeneration of motor neurons, leading to muscle weakness and atrophy. This condition is caused by a mutation in the survival motor neuron 1 (SMN1) gene, which inevitably results in a deficiency of the SMN protein. In present study, we investigated the potential role of telomere attrition in SMA patients.

From Presentation to Resolution: Documenting a Lobular Capillary Hemangioma of the Eyelid.

Lobular capillary hemangioma is a benign vascular tumor predominantly seen in pediatric populations. A 34-year-old male developed a reddish nodular mass on the upper eyelid of his left eye. An excisional biopsy was performed, and a histopathological examination of the specimen revealed proliferative vessels lined by increased endothelial cells, devoid of nuclear atypia.