The effect of polyunsaturated phosphatidylcholine on lipid synthesis in ethanol-intoxicated cultured rat hepatocytes.

Rat liver cells in primary culture have been used to study the influence of ethanol and of a polyunsaturated phosphatidylcholine preparation from soybean lecithin on glycerol incorporation into lipids. Ethanol decreases the incorporation of glycerol into choline and ethanolamine phosphoglycerides and increases that into diglyceride, either when added to the incubation medium or when administered to rats prior to hepatocyte preparation. The addition of the polyunsaturated lipid material (EPL) is able to counteract the effect of ethanol modifying the labeling of glycerol in favour of phospholipids.

The influence of polyunsaturated phosphatidylcholine on brain lipid synthesis during aging.

The synthesis of choline phosphoglycerides and ethanolamine phosphoglycerides is noticeably decreased in brain microsomal membranes of aged rats as compared to that of two-month-old rats. Experimental evidence is presented that shows that the addition of a diglyceride preparation obtained from a polyunsaturated phosphatidylcholine to microsomes of aged rats practically restores the decreased synthesizing activity of both choline and ethanolamine phosphoglycerides. It is proposed that adding diglycerides to aged membranes affects the properties of the membranes and probably enzyme-lipid interactions.

The activation of CTP: phosphocholine cytidylyltransferase from rat liver by polyunsaturated phospholipid.

Experimental evidence is reported that the addition in vitro of a polyunsaturated soybean phospholipid material (EPL) to a CTP:PC cytidylyltransferase preparation from rat liver (E.C. 2.

The influence of CDP-choline on brain lipid metabolism during ischemia.

The intraperitoneal administration of CDP-choline to gerbils (Meriones unguiculatus) partially prevented the changes of lipid metabolism found in brain after ischemia due to carotid occlusion. The increase of diglyceride pool and of its content of radioactivity, due to arachidonate labelling, was almost completely corrected by the treatment. The increase of the free fatty acids, due to ischemia, was unaltered.